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Purification of SR Protein Splicing Factors

  • Protocol
RNA-Protein Interaction Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 118))

Abstract

In recent years, the SR protein family of precursor messenger RNA splicing factors has emerged as a key player in the assembly of the spliceosomal machinery onto pre-mRNA. The SR proteins are essential splicing factors and different family members can direct usage of alternative splice sites in vitro and in vivo (reviewed in refs. 1 and 2). SR proteins are required for the earliest spliceosomal interactions in recruitment of U1snRNP (3,4), they can interact with the splicing factors that recruit U2 snRNP (5), and they appear to be essential for recruitment of the tri-snRNP to the pre-mRNA (6). When present in excess in a U1snRNP-depleted extract, they can function to bypass a U1 snRNP requirement for splicing (7,8). They have been identified as trans-acting factors that interact with cis regulatory sequences in alternatively spliced exons (3,9–13). Because they are involved in activating, enhancing, and repressing splicing for constitutive and alternatively spliced messages, they have become the focus of a great number of recent studies.

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Author information

Authors and Affiliations

  1. Department of Biology and Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California Santa Cruz, Santa Cruz, California, USA

    Alan M. Zahler

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  1. Alan M. Zahler
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Editor information

Editors and Affiliations

  1. Uniformed Services University of the Health Sciences, Bethesda, Maryland

    Susan R. Haynes

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© 1999 Humana Press Inc.

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Zahler, A.M. (1999). Purification of SR Protein Splicing Factors. In: Haynes, S.R. (eds) RNA-Protein Interaction Protocols. Methods in Molecular Biology™, vol 118. Humana Press. https://doi.org/10.1385/1-59259-676-2:419

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  • DOI: https://doi.org/10.1385/1-59259-676-2:419

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-568-3

  • Online ISBN: 978-1-59259-676-8

  • eBook Packages: Springer Protocols

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