Abstract
Proper formation of different types of ribonucleoprotein (RNP)-RNA complexes is critical for the correct expression of genetic information at the posttranscriptional level. In the nucleus, RNA polymerase II transcripts bind to an abundant class of nuclear pre-mRNA/mRNA-binding proteins, the heterogeneous nuclear ribonucleoproteins (hnRNPs), to form hnRNP complexes (1,2). The molecular architecture of these complexes must be exceedingly dynamic because each step in the pre-mRNA processing pathway alters the structure of the underlying RNA substrate. Therefore, hnRNP complexes within the cell, although probably unique for each transcript, are not uniform structures, such as a ribosomal subunit or a particular small nuclear ribonucleoprotein particle (snRNP), but are composed of a heterogeneous mixture of protein-RNA structures.
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References
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© 1999 Humana Press Inc.
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Swanson, M.S., Dreyfuss, G. (1999). Preparation of Heterogeneous Nuclear Ribonucleoprotein Complexes. In: Haynes, S.R. (eds) RNA-Protein Interaction Protocols. Methods in Molecular Biology™, vol 118. Humana Press. https://doi.org/10.1385/1-59259-676-2:299
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DOI: https://doi.org/10.1385/1-59259-676-2:299
Publisher Name: Humana Press
Print ISBN: 978-0-89603-568-3
Online ISBN: 978-1-59259-676-8
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