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Joining RNA Molecules with T4 DNA Ligase

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RNA-Protein Interaction Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 118))

Abstract

Researchers interested in studying RNA structure and function or RNA-protein interactions are increasingly using site-specifically modified RNAs to probe sites of interest (1). In addition to expanding the repertoire of functional groups beyond the very limited set available through standard mutagenesis, current synthesis techniques allow for inclusion of radioactive labels and/or various crosslinking reagents at single internal sites in RNA. The latter probes are particularly useful for determining what proteins or other RNAs interact with a site of interest on the test RNA. This chapter deals mainly with recently developed methodology for introducing such modifications into long RNAs.

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Authors and Affiliations

  1. Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, Waltham, Massachussetts, USA

    Melissa J. Moore

Authors
  1. Melissa J. Moore
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Editor information

Editors and Affiliations

  1. Uniformed Services University of the Health Sciences, Bethesda, Maryland

    Susan R. Haynes

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© 1999 Humana Press Inc.

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Moore, M.J. (1999). Joining RNA Molecules with T4 DNA Ligase. In: Haynes, S.R. (eds) RNA-Protein Interaction Protocols. Methods in Molecular Biology™, vol 118. Humana Press. https://doi.org/10.1385/1-59259-676-2:11

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  • DOI: https://doi.org/10.1385/1-59259-676-2:11

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-568-3

  • Online ISBN: 978-1-59259-676-8

  • eBook Packages: Springer Protocols

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