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Nitrocellulose Filter Binding for Determination of Dissociation Constants

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Book cover RNA-Protein Interaction Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 118))

Abstract

Nitrocellulose filter binding has been used to measure the association of proteins to both DNA and RNA for many years. Yarus and Berg (1) first described the application of the method for characterization of aminoacyl-tRNA synthetase:tRNA association; other RNA applications include the R17 coat protein:RNA hairpin interaction (2), ribosomal protein:RNA binding (3), and TFIIIA:5S (4). The method has also been extensively applied to DNA:protein systems.

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References

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri, USA

    Kathleen B. Hall & James K. Kranz

Authors
  1. Kathleen B. Hall
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  2. James K. Kranz
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Editor information

Editors and Affiliations

  1. Uniformed Services University of the Health Sciences, Bethesda, Maryland

    Susan R. Haynes

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© 1999 Humana Press Inc.

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Hall, K.B., Kranz, J.K. (1999). Nitrocellulose Filter Binding for Determination of Dissociation Constants. In: Haynes, S.R. (eds) RNA-Protein Interaction Protocols. Methods in Molecular Biology™, vol 118. Humana Press. https://doi.org/10.1385/1-59259-676-2:105

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  • DOI: https://doi.org/10.1385/1-59259-676-2:105

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-568-3

  • Online ISBN: 978-1-59259-676-8

  • eBook Packages: Springer Protocols

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