Abstract
The measurement of DNA excision repair activity in vitro, originally developed by Wood and co-workers (1), utilizes transcriptionally active protein extracts obtained from mammalian cells by the method of Manley et al. (2) (see Chapter 29). Nucleotide excision repair (NER), which requires more than 20 proteins in vitro, can process a large variety of lesions according to the following mechanism:
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1.
Recognition of the DNA lesion.
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2.
Incision on both sides of the lesion.
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3.
Excision of the damaged oligonucleotide.
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4.
DNA polymerization and ligation.
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References
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© 1999 Humana Press Inc.
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Salles, B., Calsou, P. (1999). In Vitro Excision Repair Assay in Schizosaccharomyces pombe . In: Henderson, D.S. (eds) DNA Repair Protocols. Methods in Molecular Biology™, vol 113. Humana Press. https://doi.org/10.1385/1-59259-675-4:327
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DOI: https://doi.org/10.1385/1-59259-675-4:327
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