Abstract
Viral dynamics is the study of the population dynamics of viral infection within the body of an infected individual. It describes how viruses spread from cell to cell, with the aim of revealing the basic laws that govern the spread of the virus within the host, their interaction with the immune system, and their response to therapy. For viruses that produce a viremia, changes in the size of the virus population can be measured and followed from blood samples obtained by phlebotomy at various time intervals using very sensitive polymerase chain reaction (PCR)-based assays to quantify viral nucleic acids accurately over a dynamic range of six orders of magnitude.
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References
Wei, X., Ghosh, S. K., Taylor, M. E., et al. (1995) Viral dynamics in human immunodeficiency virus type 1 infection. Nature 373, 117ā122.
Ho, D. D., Neumann, A. U., Perelson, A. S., Chen, W., Leonard, J. M., and Markowitz, M. (1995) Rapid turnover of plasma virion and CD4 lymphocytes in HIV-1 infection. Nature 373, 123ā126.
Perelson, A. S., Essunger, P., and Ho, D. D. (1997) Dynamics of HIV-1 and CD4+ lymphocytes in vivo. AIDS 11(Suppl A), S17āS24.
Perelson, A. S., Essunger, P., Cao, Y., et al. (1997) Decay characteristics of HIV-1 infected compartments during combination therapy. Nature 387, 188ā191.
Zeuzem, S., Schmidt, J. M., Lee, J.-H., von Wagner, M., Teuber, G., and Roth W. K. (1998) Hepatitis C virus dynamics in vivo: effect of ribavirin and interferon alfa on viral turnover. Hepatology 28, 245ā252.
Neumann, A. U., Lam, N. P., Dahari, H., et al. (1998) Hepatitis C viral dynamics in vivo and the antiviral efficacy of interferon-Ī± therapy. Science 282, 103ā107.
Nowak, M. A. and Bangham, C. R. M. (1996) Population dynamics of immune responses to persistent viruses. Science 272, 74ā79.
Grisham, J. W. (1962) A morphologic study of deoxyribonucleic acid synthesis and cell proliferation in regenerating rat liver: autoradiography with thymidine-H3. Cancer Res. 22, 842ā849.
Blikkendaal-Lieftinck, L. F., Kooij, M., Kramer, M. F., and Den Otter, W. (1977) Cell kinetics in the liver of rats under normal and abnormal dietary conditions. Exp. Mol. Pathol. 26, 184ā192.
Bianchi, L. and Gudat, F. (1980) Viral antigens in liver tissue and type of inflammation in hepatitis B, in Virus and the Liver. (Bianchi, L., Gerok, W., and Sickinger, K., eds.), MTP, Lancaster, UK, pp. 197ā204.
Guidotti, L. G., Rochford, R., Chung, J., Shapiro, M., Purcell, R., and Chisari, F. V. (1999) Viral clearance without destruction of infected cells during acute HBV infection. Science 284, 825ā829.
Nowak, M. A., Bonhoeffer, S., Hill, A. M., Boehme, R., Thomas, H. C., and McDade, H. (1996) Viral dynamics in hepatitis B virus infection. Proc. Natl. Acad. Sci. USA 93, 4398ā4402.
Tsiang, M., Rooney, J. F., Toole, J. J., and Gibbs, C. S. (1999) Biphasic clearance kinetics of hepatitis B virus from patients during adefovir dipivoxil therapy. Hepatology 29, 1863ā1869.
Perelson, A. S., Neumann, A. U., Markowitz, M., Leonard, J. M., and Ho, D. D. (1996) HIV-1 dynamics in vivo: virion clearance rate, infected cell life-span, and viral generation time. Science 271, 1582ā1586.
Lau, G. K. K., Tsiang, M., Hou, J., et al. (2000) Combination therapy with lamivudine and famciclovir for chronic hepatitis B-infected Chinese patients: a viral dynamics study. Hepatology 32, 394ā399.
Herz, A. V. M., Bonhoeffer, S., Anderson, R. M., Ma, R. M., and Nowak, M. A. (1996) Viral dynamics in vivo: limitations on estimates of intracellular delay and virus decay. Proc. Natl. Acad. Sci. USA 93, 7247ā7251.
Whalley, S. A., Murray, J. M., Brown, D., et al. (2001) Kinetics of acute hepatitis B virus infection in humans. J. Exp. Med. 193, 847ā854.
Nowak, M. A., Lloyd, A., Vasquez, G. M., et al. (1997) Viral dynamics of primary viremia and antiretroviral therapy in simian immunodeficiency virus infection. J. Virol. 71, 7518ā7525.
Tsiang, M., Lau, G. K. K., Cheeseman, L. Murray, A., and Gibbs, C. S. (2000) Viral dynamics analysis of HBV clearance under different antiviral treatment regimens using a mathematical model. Antiviral Res. 46, A57.
Lewin, S. R., Walters, T., Bowden, S., et al. (2001) Hepatitis B virion (HBV) clearance is extremely rapid in a subset of chronically infected individuals. Antiviral Res. 50, A80.
Zhu, Y., Yamamoto, T., Cullen, J., et al. (2001) Kinetics of hepadnavirus loss from the liver during inhibition of viral DNA synthesis. J. Virol. 75, 311ā322.
Liaw, Y.-F., Leung, N. W. Y., Chang, T.-T., et al. (2000) Effect of extended lamivudine therapy in Asian patients with chronic hepatitis B. Gastroenterology 119, 172ā180.
Westland, C. E., Yang, H., Delaney IV, W. E., et al. (2001) Week 48 resistance surveillance in chronic hepatitis B patients enrolled in a phase III clinical study of adefovir dipivoxil. Hepatology 34(Suppl 4), 447A.
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Tsiang, M., Gibbs, C.S. (2004). Analysis of Hepatitis B Virus Dynamics and Its Impact on Antiviral Development. In: Hamatake, R.K., Lau, J.Y.N. (eds) Hepatitis B and D Protocols. Methods in Molecular Medicineā¢, vol 96. Humana Press. https://doi.org/10.1385/1-59259-670-3:361
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DOI: https://doi.org/10.1385/1-59259-670-3:361
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