Abstract
Establishing novel in vitro model systems for studying hepatitis B virus (HBV) pathogenesis and the effects of antivirals on HBV replication is necessary because of the limitations encountered studying HBV in vivo. However, there are many challenges to in vitro studies as well.
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Sells, M.A., Chen, M.L., and Acs, G. (1987) Production of hepatitis B virus particles in Hep G2 cells transfected with cloned hepatitis B virus DNA. Proc. Natl. Acad. Sci. USA 84, 1005ā1009.
Newbold, J.E., Xin, H., Tencza, M., et al. (1995) The covalently closed duplex form of the hepadnavirus genome exists in situ as a heterogeneous population of viral minichromosomes. J. Virol. 69, 3350ā3357.
Wood, H.A. and Granados, R.R. (1991) Genetically engineered baculoviruses as agents for pest control. Annu. Rev. Microbiol. 45, 69ā87.
Smith, G.E., Summers, M.D., and Fraser, M.J. (1983) Production of human beta interferon in insect cells infected with a baculovirus expression vector. Mol. Cell. Biol. 3, 2156ā2165.
Pennock, G.D., Shoemaker, C., and Miller, L.K. (1984) Strong and regulated expression of Escherichia coli beta galactosidase in insect cells with a baculovirus vector. Mol. Cell. Biol. 4, 399ā406.
Hofmann, C., Sandig, V., Jennings, G., Rudolph, M., Schlag, P., and Strauss, M. (1995) Efficient gene transfer into human hepatocytes by baculovirus vectors. Proc. Natl. Acad. Sci. USA 92, 10099ā10103.
Sandig, V., Hofmann, C., Steinert, S., Jennings, G., Schlag, P., and Strauss, M. (1996) Gene transfer into hepatocytes and human liver tissue by baculovirus vectors. Hum. Gene Ther. 7, 1937ā1945.
Boyce, F.M. and Bucher, N.L. (1996) Baculovirus-mediated gene transfer into mammalian cells. Proc. Natl. Acad. Sci. USA 93, 2348ā2352.
Bilello, J.P., Delaney, W.E.IV, Boyce, F.M., and Isom, H.C. (2001) Transient disruption of intracellular junctions enables baculovirus entry into nondividing hepatocytes. J. Virol. 75, 9857ā9871.
Shoji, I., Aizaki, H., Tani, H., et al. (1997) Efficient gene transfer into various mammalian cells, including non-hepatic cells, by baculovirus vectors. J. Gen. Virol. 78, 2657ā2664.
Delaney, W.E. IV and Isom, H.C. (1998) Hepatitis B replication in human HepG2 cells mediated by hepatitis B virus recombinant baculovirus. Hepatology 28, 1134ā1146.
Delaney, W.E. IV, Miller, T.G., and Isom, H.C. (1999) Use of the hepatitis B virus recombinant baculovirus-HepG2 system to study the effects of (ā)-Ī²-2ā²,3ā²-dideoxy-3ā²-thiacytidine on replication of hepatitis B virus and accumulation of covalently closed circular DNA. Antimicrob. Agents Chemother. 43, 2017ā2026.
Abdelhamed, A.M., Kelley, C.M., Miller, T.G., Furman, P.A., and Isom, H.C. (2002) Rebound of hepatitis B virus replication in HepG2 cells after cessation of antiviral treatment. J. Virol. 76, 8148ā8160.
Delaney, W.E. IV, Edwards, R., Colledge, D., et al. (2001) Cross-resistance testing of anti-hepadnaviral compounds using novel recombinant baculoviruses which encode drug-resistant strains of hepatitis B virus. Antimicrob. Agents Chemother. 45, 1705ā1713.
Abdelhamed, A.M., Kelley, C.M., Miller, T.G., Cable, E.E., Furman, P.A., and Isom, H.C. (2003) Comparison of the anti-hepatitis B virus activity of lamivudine and clevudine using a quantitative assay. Antimicrob. Agents Chemother. 47, 324ā336.
Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Analysis and cloning of eukaryotic genomic DNA, In Molecular Cloning: A Laboratory Manual 2nd edit. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 9.1ā9.62.
Ausubel, F.M., Brent, R., Kingston, R.E., et al. (2002) In Current Protocols in Molecular Biology. John Wiley & Sons, New York.
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Isom, H.C., Abdelhamed, A.M., Bilello, J.P., Miller, T.G. (2004). Baculovirus-Mediated Gene Transfer for the Study of Hepatitis B Virus. In: Hamatake, R.K., Lau, J.Y.N. (eds) Hepatitis B and D Protocols. Methods in Molecular Medicineā¢, vol 96. Humana Press. https://doi.org/10.1385/1-59259-670-3:219
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DOI: https://doi.org/10.1385/1-59259-670-3:219
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