Abstract
Hepatitis B viruses (HBVs) are characterized by their high species and tissue specificity. Because of the large number of patients chronically infected with HBV, the development of new treatment strategies remains a major goal but is hindered by the lack of infection systems that would allow testing. Only primary human or humanoid hepatocytes are fully permissive for HBV infection. Consequently, no permissive cell line and no convenient small animal model are available to study HBV infection. However, highly differentiated liver cell lines support virus replication following transfection of replication competent HBV constructs. Stable cell lines with integrated HBV genomes, for example, HepG 2.2.15 cells (1), are commonly used for assessing the action of drugs on HBV replication. HBV-transgenic mice (2) and have been generated and were proven to be very useful for immunological studies. However, stable cell lines as well as transgenic mice, unlike in natural infection, replicate HBV from an integrated genome that cannot be eliminated. In addition, they cannot mimic an acute infection and the level of virus replication cannot be varied or adjusted.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Acs, G., Sells, M. A., Purcell, R.H., et al. (1987) Hepatitis B virus produced by transfected Hep G2 cells causes hepatitis in chimpanzees. Proc. Natl. Acad. Sci. USA 84, 4641–4644.
Guidotti, L.G., Matzke, B., Schaller, H., and Chisari, F.V. (1995) High-level hepatitis B virus replication in transgenic mice. J. Virol. 69, 6158–6159.
Delaney IV, W. E. and Isom, H. C. (1998) Hepatitis B virus replication in human HepG2 cells mediated by hepatitis B virus recombinant baculovirus. Hematology 28, 1134–1146.
Sprinzl, M.F., Oberwinkler, H., Schaller, H., and Protzer, U. (2001) Transfer of hepatitis B virus genome by adenovirus vectors into cultured cells and mice: crossing the species barrier. J. Virol. 75, 5108–5118.
Ren, S. and Nassal, M. (2001) Hepatitis B virus (HBV) virion and covalently closed circular DNA formation in primary tupaia hepatocytes and human hepatoma cell lines upon HBV genome transduction with replication-defective adenovirus vectors. J. Virol. 75, 1104–1116.
Graham, F. L., Smiley, J., Russel, W. C, and Nairn, R. (1977) Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36, 59–72.
He, T.C., Zhou, S., da Costa, L. T., Yu, J., Kinzler, K.W. and Vogelstein, B. (1998) A simplified system for generating recombinant adenoviruses. Proc. Natl. Acad. Sci. USA 95, 2509–2514.
Fallaux, F.J., Bout, A., van der Velde, I., et al. (1998) New helper cells and matched early region 1-deleted adenovirus vectors prevent generation of replication-competent adenoviruses. Hum. Gene Ther. 9, 1909–1917.
Will, H., Cattaneo, R., Darai, G., Deinhardt, F., Schellekens, H., and Schaller, H. (1985) Infectious hepatitis B virus from cloned DNA of known nucleotide sequence. Proc. Natl. Acad. Sci. USA 82, 891–895.
Yang, P. L., Althage, A., Chung, J., and Chisari, F. V. (2002) Hydrodynamic infection of viral DNA: a mouse model of acute hepatitis B virus infection. Proc. Natl. Acad. Sci. USA 21, 13825–13830.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2004 Humana Press Inc.
About this protocol
Cite this protocol
Sprinzl, M., Dumortier, J., Protzer, U. (2004). Construction of Recombinant Adenoviruses that Produce Infectious Hepatitis B Virus. In: Hamatake, R.K., Lau, J.Y.N. (eds) Hepatitis B and D Protocols. Methods in Molecular Medicine™, vol 96. Humana Press. https://doi.org/10.1385/1-59259-670-3:209
Download citation
DOI: https://doi.org/10.1385/1-59259-670-3:209
Publisher Name: Humana Press
Print ISBN: 978-1-58829-108-0
Online ISBN: 978-1-59259-670-6
eBook Packages: Springer Protocols