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Hepatitis B and D Protocols pp 85–93Cite as

Detection of Serum HDV RNA by RT-PCR

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Part of the book series: Methods in Molecular Medicine ((MIMM,volume 95))

Abstract

The cloning and sequencing of the hepatitis D virus (HDV) genome in 1986 established the peculiar features that make HDV the only genus of the Deltaviridae family (1). HDV is a defective negative-strand RNA virus that requires concurrent infection with the hepatitis B virus (HBV) to complete its replicative cycle. The 36-nm virion consists of the delta antigen (HDV Ag) and the RNA genome of 1.6 kb within an envelop of surface antigen (HBsAg), provided by the HBV infecting the host (2, Fig. 1). The structure and biological properties and replication mechanism of HDV are similar to those of plant RNA viruses such as the viroids and satellite RNAs (3).

Schematic virion of HDV.

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Author information

Authors and Affiliations

  1. Department of Gastroenterology, Casa Sollievo della Sofferenza, San Giovanni Rotondo, FG, Italy

    Antonina Smedile, Maria Grazia Niro & Mario Rizzetto

Authors
  1. Antonina Smedile
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  2. Maria Grazia Niro
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  3. Mario Rizzetto
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Editor information

Editors and Affiliations

  1. Ribapharm Inc., Costa Mesa, CA

    Robert K. Hamatake PhD  & Johnson Y. N. Lau MD  & 

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© 2004 Humana Press Inc.

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Smedile, A., Niro, M.G., Rizzetto, M. (2004). Detection of Serum HDV RNA by RT-PCR. In: Hamatake, R.K., Lau, J.Y.N. (eds) Hepatitis B and D Protocols. Methods in Molecular Medicine, vol 95. Humana Press. https://doi.org/10.1385/1-59259-669-X:85

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  • DOI: https://doi.org/10.1385/1-59259-669-X:85

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-105-9

  • Online ISBN: 978-1-59259-669-0

  • eBook Packages: Springer Protocols

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