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A cis/trans Genetic Test for Pleiotropic Phenotypes Associated with a Frequent Naturally Occurring Mutation at Amino Acid 97 of HBV Core Protein

  • Protocol
Hepatitis B and D Protocols

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 95))

Abstract

Hepatitis B virus (HBV) is a major human infectious pathogen. Worldwide, there are more than 350 million chronic carriers of HBV. Sequence divergence within the HBV genome is observed during long-term infection of HBV in patients as a result of the low fidelity of HBV reverse transcriptase. The important roles played by naturally occurring HBV variants have been implicated in the development of chronic hepatitis B. However, functional characterization of HBV variants remains difficult because of the lack of a priori knowledge to predict the possible abnormality of variants and to choose the relevant functional assay accordingly. This issue is further complicated by the possibility that a mutant phenotype could arise from either the trans defect of a mutant protein or the cis defect of a mutated genome.

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Author information

Authors and Affiliations

  1. Departments of Pathology, and Microbiology & Immunology, WHO Collaborating Center for Tropical Diseases and Sealy Center for Vaccine Developments, University of Texas Medical Branch, Galveston, TX

    Chiaho Shih & Ta-Tung Thomas Yuan

Authors
  1. Chiaho Shih
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  2. Ta-Tung Thomas Yuan
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Editor information

Editors and Affiliations

  1. Ribapharm Inc., Costa Mesa, CA

    Robert K. Hamatake PhD  & Johnson Y. N. Lau MD  & 

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© 2004 Humana Press Inc.

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Shih, C., Yuan, TT.T. (2004). A cis/trans Genetic Test for Pleiotropic Phenotypes Associated with a Frequent Naturally Occurring Mutation at Amino Acid 97 of HBV Core Protein. In: Hamatake, R.K., Lau, J.Y.N. (eds) Hepatitis B and D Protocols. Methods in Molecular Medicine, vol 95. Humana Press. https://doi.org/10.1385/1-59259-669-X:247

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  • DOI: https://doi.org/10.1385/1-59259-669-X:247

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-105-9

  • Online ISBN: 978-1-59259-669-0

  • eBook Packages: Springer Protocols

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