Skip to main content

Nucleoprotein Transport of HBV Capsid Particles

  • Protocol
Hepatitis B and D Protocols

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 95))

  • 536 Accesses

Abstract

Eukaryotic cells are compartmentalized and therefore possess mechanisms to transport molecules between the different organelles. Viruses take advantage of cellular transcription and translation machinery for their multiplication. Consequently, they utilize cellular routes for transporting their proteins and genome into the correct compartments.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Seeger, C. and Mason, W. S. (2000) Hepatitis B virus biology. Microbiol. Mol. Biol. Rev. 64, 51–68.

    Article  PubMed  CAS  Google Scholar 

  2. Tuttleman, J. S., Pugh, J. C., and Summers, J. W. (1986) In vitro experimental infection of primary duck hepatocyte cultures with duck hepatitis B virus. J. Virol. 58, 17–25.

    PubMed  CAS  Google Scholar 

  3. Adam, S. A., Marr, R. S., and Gerace, L. (1990) Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors. J. Cell. Biol. 111, 807–816.

    Article  PubMed  CAS  Google Scholar 

  4. Görlich, D. and Kutay, U. (1999) Transport between the cell nucleus and the cytoplasm. Annu. Rev. Cell. Dev. Biol. 15, 607–660.

    Article  PubMed  Google Scholar 

  5. Whittaker, G. R., Kann, M., and Helenius, A. (2000) Viral entry into the nucleus. Annu. Rev. Cell. Dev. Biol. 16, 627–657.

    Article  PubMed  CAS  Google Scholar 

  6. Pante, N. and Kann, M. (2002) Nuclear pore complex is able to transport macromolecules with diameters of ∼39 nm. Mol. Biol. Cell. 13, 425–434.

    Article  PubMed  CAS  Google Scholar 

  7. Kann, M., Bischof, A., and Gerlich, W. H. (1997) In vitro model for the nuclear transport of the hepadnavirus genome. J. Virol. 71.

    Google Scholar 

  8. Kann, M., Sodeik, B., Vlachou, A., Gerlich, W. H., and Helenius, A. (1999) Phosphorylation-dependent binding of hepatitis B virus core particles to the nuclear pore complex. J. Cell. Biol. 145, 45–55.

    Article  PubMed  CAS  Google Scholar 

  9. Mabit, H., Breiner, K. M., Knaust, A., Zachmann-Brand, B., and Schaller, H. (2001) Signals for bidirectional nucleocytoplasmic transport in the duck hepatitis B virus capsid protein. J. Virol. 75, 1968–1977.

    Article  PubMed  CAS  Google Scholar 

  10. Görlich, D., Prehn, S., Laskey, R. A., and Hartmann, E. (1994) Isolation of a protein that is essential for the first step of nuclear protein import. Cell 79, 767–778.

    Article  PubMed  Google Scholar 

  11. Siomi, M. C., Fromont, M., Rain, J. C., et al. (1998) Functional conservation of the transportin nuclear import pathway in divergent organisms. Mol. Cell. Biol. 18, 4141–4148.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2004 Humana Press Inc.

About this protocol

Cite this protocol

Kann, M. (2004). Nucleoprotein Transport of HBV Capsid Particles. In: Hamatake, R.K., Lau, J.Y.N. (eds) Hepatitis B and D Protocols. Methods in Molecular Medicine, vol 95. Humana Press. https://doi.org/10.1385/1-59259-669-X:213

Download citation

  • DOI: https://doi.org/10.1385/1-59259-669-X:213

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-105-9

  • Online ISBN: 978-1-59259-669-0

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics