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Detection of Cytokine- and Chemokine-Expressing Cells at the Single Cell Level

  • Protocol
Book cover Cytokine Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 249))

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Abstract

Methods for detection of cytokines and chemokines at a protein level in individual cells based on immunofluorescence or immunohistochemical (1–16) staining techniques have been developed in our laboratory. The difficulties in these procedures lie in the critical steps of fixation and permeabilization of the cells in combination with a very careful selection of suitable cytokine- or chemokine-specific antibodies.

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Authors and Affiliations

  1. Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden

    Karin Loré & Jan Andersson

Authors
  1. Karin Loré
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  2. Jan Andersson
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Editors and Affiliations

  1. Laboratory of Biochemistry, Department of Chemistry, Katholieke Universiteit Leuven, Leuven, Belgium

    Marc De Ley

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© 2004 Humana Press Inc., Totowa, NJ

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Loré, K., Andersson, J. (2004). Detection of Cytokine- and Chemokine-Expressing Cells at the Single Cell Level. In: De Ley, M. (eds) Cytokine Protocols. Methods in Molecular Biology, vol 249. Humana Press. https://doi.org/10.1385/1-59259-667-3:201

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  • DOI: https://doi.org/10.1385/1-59259-667-3:201

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-948-3

  • Online ISBN: 978-1-59259-667-6

  • eBook Packages: Springer Protocols

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