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Assay System for the Effect of Prostaglandin E2 in the Determination of Polarized Cytokine Production

  • Etsushi Kuroda
  • Uki Yamashita
Part of the Methods in Molecular Biology book series (MIMB, volume 249)

Abstract

The activation of CD4+ T-cell subsets, Th1 and Th2, is one of the most important focuses of immunology. Th1 and Th2 are differentiated from naive T cells after antigenic stimulation by the influence of cytokines [interleukin 12 (IL-12)/interferon-γ (IFN-γ) in Th1 and IL-4/IL-13 in Th2] or costimulatory molecules (CD80/CD86) provided from antigen presenting cells (APC), and have several important immunological functions (1). Recently, several reports have shown that chemical mediators, such as prostaglandins (PG) and histamine, also affect Th1/Th2 cytokine production and Th differentiation (2, 3, 4, 5, 6, 7). PGE2 is one of prostanoids produced by action of the enzyme cyclooxygenase (COX) on arachidonic acid. PGE2 is mainly produced by APC: monocytes, macrophages, and dendritic cells in the immune system (2). PGE2 has a suppressive effect on Th1-related cytokine productions such as IL-12 and IFN-γ by T cells, NK cells, and APCs, and an enhancing effect on Th2 development and activation.

Keywords

Spleen Cell Chemokine Production Mouse Spleen Cell Disodium Hydrogen Phosphate Alkaline Phosphatase Substrate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  • Etsushi Kuroda
    • 1
  • Uki Yamashita
    • 1
  1. 1.Department of Immunology, School of MedicineUniversity of Occupational and Environmental HealthKitakyushuJapan

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