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Antibody Engineering pp 431–441Cite as

Characterization of Antibody-Antigen Interactions by Fluorescence Spectroscopy

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 248))

Abstract

Fluorescence spectroscopy is a widely used technique for the characterization of molecular interactions in biological systems. It is highly sensitive, and allows measurements at low sample concentrations (down to 10-7 M). It is also available at a reasonably low equipment cost. These features make fluorescence spectroscopy an attractive technique as compared to other forms of optical spectroscopy.

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References

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Author information

Authors and Affiliations

  1. Department of Chemistry, The Open University, Milton Keynes, UK

    Sotiris Missailidis

  2. School of Chemistry, University of Nottingham, Nottingham, UK

    Kevin Brady

Authors
  1. Sotiris Missailidis
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  2. Kevin Brady
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Editor information

Editors and Affiliations

  1. MRC Laboratory of Molecular Biology, University of Cambridge, Cambridge, UK

    Benny K. C. Lo

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© 2004 Humana Press Inc., Totowa, NJ

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Missailidis, S., Brady, K. (2004). Characterization of Antibody-Antigen Interactions by Fluorescence Spectroscopy. In: Lo, B.K.C. (eds) Antibody Engineering. Methods in Molecular Biology™, vol 248. Humana Press. https://doi.org/10.1385/1-59259-666-5:431

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  • DOI: https://doi.org/10.1385/1-59259-666-5:431

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-092-2

  • Online ISBN: 978-1-59259-666-9

  • eBook Packages: Springer Protocols

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