Polytene Chromosomes From Ovarian Nurse Cells of Drosophila melanogaster otu Mutants

  • Dmitry E. Koryakov
  • Natalia I. Maľceva
  • Robert C. King
  • Igor F. Zhimulev
Part of the Methods in Molecular Biology book series (MIMB, volume 247)

Abstract

Polytene chromosomes from the salivary gland cells of Drosophila melanogaster larvae have been the most useful model for studying the functional morphology of interphase eukaryotic chromosomes since their discovery almost 70 yr ago (1). The question remains as to whether the polytene structure is universal. How similar is the organization of polytene chromosomes in different tissues, because cell differentiation may lead to diverse functional chromosome states? The greatest difference can be expected to be seen between the chromosomes of the germ-line cells and the cells of somatic tissue.

Keywords

Glycerol Corn Tungsten CaCl2 Compaction 

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Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  • Dmitry E. Koryakov
    • 1
  • Natalia I. Maľceva
    • 2
  • Robert C. King
    • 3
  • Igor F. Zhimulev
    • 2
  1. 1.Department of Cytology and GeneticsNovosibirsk State UniversityNovosibirskRussia
  2. 2.Laboratory of Molecular Cytogenetics, Institute of Cytology and GeneticsSiberian Branch of the Russian Academy of ScienceNovosibirskRussia
  3. 3.Department of Biochemistry, Molecular Biology and Cell BiologyNorthwestern UniversityEvanston

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