Abstract
Control of pH is a central consideration in handling proteins, and this requires the use of buffer solutions. Furthermore, a typical protein purification schedule will contain several steps generally needed to be carried out in buffers of different pH values and ionic strengths (see Note 1). Hence, it is necessary to have available methods for changing buffers. The present chapter reviews the properties of buffers and preparation of buffer solutions and then deals with ways in which a protein solution can be changed from one buffer to another.
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References
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© 2004 Humana Press Inc., Totowa, NJ
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Doonan, S. (2004). Making and Changing Buffers. In: Cutler, P. (eds) Protein Purification Protocols. Methods in Molecular Biology, vol 244. Humana Press. https://doi.org/10.1385/1-59259-655-X:91
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DOI: https://doi.org/10.1385/1-59259-655-X:91
Publisher Name: Humana Press
Print ISBN: 978-1-58829-067-0
Online ISBN: 978-1-59259-655-3
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