Abstract
Modern liquid chromatography (LC), commonly referred to as high-performance liquid chromatography (HPLC), is over 30 yr old. Because of its age, many people might consider HPLC a mature technique and, as such, view the field as stagnant. However, nothing could be further from reality. The field of HPLC is changing rapidly although more subtly than it did in earlier years and it is interesting to note that today’s trends are in essence driven by the same application benefits that were prevalent in the early years of LC (viz. improved quantitation, rapid determination of purity and structure, and preconcentration of samples). HPLC has gained wide acceptance in the modern analytical laboratory as well as in most other areas of science.
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© 2004 Humana Press Inc., Totowa, NJ
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Neville, W.A. (2004). Reversed-Phase Chromatography of Proteins. In: Cutler, P. (eds) Protein Purification Protocols. Methods in Molecular Biology, vol 244. Humana Press. https://doi.org/10.1385/1-59259-655-X:259
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DOI: https://doi.org/10.1385/1-59259-655-X:259
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