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Protein Purification Protocols pp 205–223Cite as

Affinity Precipitation Methods

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 244))

Abstract

Affinity chromatography (see Chapter 16) is a powerful protein purification technique that exploits the specific interaction between a biological ligand (e.g., a substrate, coenzyme, hormone, antibody, or nucleic acid) or its synthetic analog and its complementary binding site on a protein. One of the variations on this technique (see refs. 1 and 2 for reviews) was that of affinity precipitation. As in affinity chromatography, the protein binds to a specific ligand, but the latter is free in solution, rather than bound to an insoluble support. Ligand binding results in the precipitation of the protein, which may then be separated by centrifugation. The pellet contains the protein of interest and the ligand, whereas the other components of the mixture remain in the supernatant, allowing easy separation.

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Author information

Authors and Affiliations

  1. Department of Veterinary Physiology and Biochemistry, University College Dublin, Dublin, Ireland

    Jane A. Irwin

  2. Department of Biochemistry, Trinity College, Dublin, Ireland

    Keith F. Tipton

Authors
  1. Jane A. Irwin
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  2. Keith F. Tipton
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Editor information

Editors and Affiliations

  1. Genomic and Proteomic Sciences Medicines Research Center, GlaxoSmithKline Pharmaceuticals, Stevenage, UK

    Paul Cutler

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© 2004 Humana Press Inc., Totowa, NJ

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Irwin, J.A., Tipton, K.F. (2004). Affinity Precipitation Methods. In: Cutler, P. (eds) Protein Purification Protocols. Methods in Molecular Biology, vol 244. Humana Press. https://doi.org/10.1385/1-59259-655-X:205

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  • DOI: https://doi.org/10.1385/1-59259-655-X:205

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-067-0

  • Online ISBN: 978-1-59259-655-3

  • eBook Packages: Springer Protocols

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