Abstract
Ion-exchange chromatography is one of the most widely used forms of column chromatography. It is used in research, analysis, and process-scale purification of proteins. Ion exchange is ideal for initial capture of proteins because of its high capacity, relatively low cost, and its ability to survive rigorous cleaning regimes. Ion exchange is also ideal for “polishing” of partially purified material on account of the high-resolution attainable and the high capacity giving the ability to achieve a high concentration of product. Ion-exchange chromatography is widely applicable because the buffer conditions can be adapted to suit a broad range of proteins rather than being applicable to a single functional group of proteins.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Amersham Pharmacia Biotech (1999) Ion Exchange Chromatography Principles and Methods, Amersham Pharmacia Biotech, Uppsala, Sweden.
Righetti, P. G. and Carravaggio, T. (1976) Isoelectric points and molecular weights of proteins: a table. J. Chromatogr. 127, 1–28.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2004 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Selkirk, C. (2004). Ion-Exchange Chromatography. In: Cutler, P. (eds) Protein Purification Protocols. Methods in Molecular Biology, vol 244. Humana Press. https://doi.org/10.1385/1-59259-655-X:125
Download citation
DOI: https://doi.org/10.1385/1-59259-655-X:125
Publisher Name: Humana Press
Print ISBN: 978-1-58829-067-0
Online ISBN: 978-1-59259-655-3
eBook Packages: Springer Protocols