Two-Dimensional Polyarcylamide Gel Electrophoresis for Proteome Analyses
Part of the Methods in Molecular Biology book series (MIMB, volume 244)
The term “proteome” can be defined, in an analogous manner to the term “genome,” as the complement of the proteins in a biological system. Undertaking a proteomic analysis enables the identification and quantitation of proteins on a proteomewide scale, thereby allowing comparisons between different proteomes. The current methodology of choice for proteome analysis is based on protein separation by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). 2-D PAGE technology, by virtue of differences in protein charge and molecular mass, allows the resolution of many thousands of proteins at the same time (see Fig. 1).
KeywordsAmersham Bioscience Sample Preparation Procedure Protein Separation Tributyl Phosphine Genomic Solution
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© Humana Press Inc., Totowa, NJ 2004