Two-Dimensional Polyarcylamide Gel Electrophoresis for Proteome Analyses

  • Neil A. Jones
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 244)

Abstract

The term “proteome” can be defined, in an analogous manner to the term “genome,” as the complement of the proteins in a biological system. Undertaking a proteomic analysis enables the identification and quantitation of proteins on a proteomewide scale, thereby allowing comparisons between different proteomes. The current methodology of choice for proteome analysis is based on protein separation by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). 2-D PAGE technology, by virtue of differences in protein charge and molecular mass, allows the resolution of many thousands of proteins at the same time (see Fig. 1).
Fig. 1.

Typical two-dimensional sodium dodecyl sulfate-polyacrylamide gel. A total cellular protein extract from Saccharomyces cerevisiae was separated using pH 4–7 immobilized pH gradient (IPG) strips in the first dimension and 12% SDS-PAGE in the second dimension. Proteins were visualized by silver staining.

Keywords

Glycerol Urea Glycine Dehydration Butanol 

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Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  • Neil A. Jones
    • 1
  1. 1.Genomic and Proteomic Sciences, Medicines Research CenterGlaxoSmithKline PharmaceuticalsStevenageUK

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