Abstract
Vectors derived from retroviruses have been widely studied as tools for gene transfer into mammalian tissue in vivo. One application for which retroviral vectors have received particular attention is gene transfer into tumor cells for treatment of cancer. Simple retroviruses, such as murine leukemia virus (MLV), and the vectors derived from them, require cell division for infection and thus possess a degree of inherent specificity for the rapidly dividing cells of neoplastic tissue. This unique property and the ease with which retroviral vectors are manipulated and produced have provided much of the impetus for their use in experimental and clinical cancer gene-therapy studies.
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Burns, J. C., Friedmann, T., Driever, W., Burrascano, M., and Yee, J. K. (1993) Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells. Proc. Natl. Acad. Sci. USA 90, 8033–8037.
Cohen, L. A. (1982) Isolation and characterization of a serially cultivated, neoplastic, epithelial cell line from the N-nitrosomethylurea induced rat mammary adenocarcinoma. In Vitro 18, 565–575.
Cornetta, K., Moen, R. C., Culver, K., Morgan, R. A., McLachlin, J. R., Sturm, S., et al. (1990) Amphotropic murine leukemia retrovirus is not an acute pathogen for primates. Hum. Gene Ther. 1, 15–30.
Culver, K. W., Ram, Z., Wallbridge, S., Ishii, H., Oldfield, E. H., and Blaese, R. M. (1992) In vivo gene transfer with retroviral vector-producer cells for treatment of experimental brain tumors. Science 256, 1550–1552.
Diaz, R. M., Eisen, T., Hart, I. R., and Vile, R. G. (1998) Exchange of viral promoter/enhancer elements with heterologous regulatory sequences generates targeted hybrid long terminal repeat vectors for gene therapy of melanoma. J. Virol. 72, 789–795.
Donahue, R. E., Kessler, S. W., Bodine, D., McDonagh, K., Dunbar, C, Goodman, S., et al. (1992) Helper virus induced T cell lymphoma in nonhuman primates after retroviral mediated gene transfer. J. Exp. Med. 176, 1125–1135.
DuBridge, R. B., Tang, P., Hsia, H. C., Leong, P. M., Miller, J. H., and Calos, M. P. (1987) Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system. Mol. Cell Biol. 7, 379–387.
Ezzeddine, Z. D., Martuza, R. L., Platika, D., Short, M. P., Malick, A., Choi, B.,and Breakefield, X. O. (1991) Selective killing of glioma cells in culture and in vivo by retrovirus transfer of the herpes simplex virus thymidine kinase gene. New Biol. 3, 608–614.
Han, J. Y., Zhao, Y., Anderson, W. F., and Cannon, P. M. (1998) Role of variable regions A and B in receptor binding domain of amphotropic murine leukemia virus envelope protein. J. Virol. 72, 9101–9108.
Han, X., Kasahara, N., and Kan, Y. W. (1995) Ligand-directed retroviral targeting of human breast cancer cells. Proc. Natl. Acad. Sci. USA 92, 9747–9751.
Liu, X., Constantinescu, S. N., Sun, Y., Bogan, J. S., Hirsch, D., Weinberg, R. A., and Lodish, H. F. (2000) Generation of mammalian cells stably expressing multiple genes at predetermined levels. Anal. Biochem. 280, 20–28.
Logg, C. R., Logg, A., Matusik, R. J., Bochner, B. H., and Kasahara, N. (2002) Tissue-specific transcriptional targeting of a replication-competent retroviral vector. J. Virol. 76, 12783–12791.
Logg, C. R., Logg, A., Tai, C. K., Cannon, P. M., and Kasahara, N. (2001) Genomic stability of murine leukemia viruses containing insertions at the Env-3′ untranslated region boundary. J. Virol. 75, 6989–6998.
Logg, C. R., Tai, C. K., Logg, A., Anderson, W. F., and Kasahara, N. (2001) A uniquely stable replication-competent retrovirus vector achieves efficient gene delivery in vitro and in solid tumors. Hum. Gene Ther. 12, 921–932.
Miller, D. G., Adam, M. A., and Miller, A. D. (1990) Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection [published erratum appears in Mol Cell Biol 1992 Jan;12(1)∶433]. Mol. Cell Biol. 10, 4239–4242.
Naviaux, R. K., Costanzi, E., Haas, M., and Verma, I. M. (1996) The pCL vector system: rapid production of helper-free, high-titer, recombinant retroviruses. J. Virol. 70, 5701–5705.
Ory, D. S., Neugeboren, B. A., and Mulligan, R. C. (1996) A stable human-derived packaging cell line for production of high titer retrovirus/vesicular stomatitis virus G pseudotypes. Proc. Natl. Acad. Sci. USA 93, 11400–11406.
Rainov, N. G. (2000) A phase III clinical evaluation of herpes simplex virus type 1 thymidine kinase and ganciclovir gene therapy as an adjuvant to surgical resection and radiation in adults with previously untreated glioblastoma multiforme. Hum. Gene Ther. 11, 2389–2401.
Ram, Z., Walbridge, S., Shawker, T., Culver, K. W., Blaese, R. M., and Oldfield, E. H. (1994) The effect of thymidine kinase transduction and ganciclovir therapy on tumor vasculature and growth of 9L gliomas in rats. J. Neurosurg. 81, 256–60.
Soneoka, Y., Cannon, P. M., Ramsdale, E. E., Griffiths, J. C., Romano, G., Kingsman, S. M., and Kingsman, A. J. (1995) A transient three-plasmid expression system for the production of high titer retroviral vectors. Nucleic Acids Res. 23, 628–633.
Parmar, M. K. B. and Machin, D. (1995) Survival Analysis: A Practical Approach. John Wiley and Sons, Chichester, UK.
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© 2004 Humana Press Inc., Totowa, NJ
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Logg, C.R., Kasahara, N. (2004). Retrovirus-Mediated Gene Transfer to Tumors. In: Heiser, W.C. (eds) Gene Delivery to Mammalian Cells. Methods in Molecular Biology™, vol 246. Humana Press. https://doi.org/10.1385/1-59259-650-9:499
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DOI: https://doi.org/10.1385/1-59259-650-9:499
Publisher Name: Humana Press
Print ISBN: 978-1-58829-095-3
Online ISBN: 978-1-59259-650-8
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