Baculovirus-Mediated Gene Delivery into Mammalian Cells

  • Raymond V. Merrihew
  • Thomas A. Kost
  • J. Patrick Condreay
Part of the Methods in Molecular Biology™ book series (MIMB, volume 246)


A relatively recent advance in the use of recombinant baculoviruses is their use for delivery of genes and genetic elements into mammalian cells. Baculovirus vectors retrofitted with mammalian gene promoters have been shown to efficiently deliver and express genes in a broad assortment of cell types. These baculovirus transductions are simple to perform, reproducible, and demonstrate no overt cell toxicity. Baculovirus-mediated gene delivery is particularly useful for repetitive or moderately high-throughput procedures such as cell-based assays, or for situations where transfection procedures are inadequate.


Sodium Butyrate Recombinant Baculovirus Virus Inoculum Recombinant Baculoviruses Baculovirus Vector 
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Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  • Raymond V. Merrihew
    • 1
  • Thomas A. Kost
    • 2
  • J. Patrick Condreay
    • 2
  1. 1.Department of Assay Development and Compound ProfilingGlaxoSmithKlineResearch Triangle Park
  2. 2.Department of Gene Expression and Protein BiochemistryGlaxoSmithKline Research and DevelopmentResearch Triangle Park

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