Biolistic Transfection of Cultured Organotypic Brain Slices
Transfection of postmitotic neurons remains one of the most challenging goals in the field of gene delivery. For many cell types, gene transfer using the various methods described in this book has become a routine tool for studying gene regulation and function. However, postmitotic neurons are not easily transfected by most gene-delivery methods. Conventional techniques to transfect neurons in intact tissue are problematic because they are often unreliable or too time-consuming.
KeywordsGold Particle Slice Culture Cortical Slice Prep Station Postmitotic Neuron