Abstract
Atomic force microscopy (AFM) has been successfully applied not only to the topography of biological specimens but also to the measurement of their local mechanical properties. This technique is very useful for imaging such biological specimens as cells, proteins, and DNA, because no special treatments of samples are required (1,2). The measurement of local mechanical properties in a living cell is achieved with a nanoindentation technique (3). The method has been applied to several kinds of cultured cells, including Madin-Darby canine kidney (MDCK) cells (4), myocytes (5), fibroblasts (6,7), and endothelial cells (8,9). However, morphology and properties are different between cells cultured in vitro on substrate and intact cells in vivo (10).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Hansma, H. G. and Hoh, J. H. (1994) Biomolecular imaging with the atomic force microscope. Annu. Rev. Biophys. Biomol. Struct. 23, 115–139.
Lal, R. and John, S. A. (1994) Biological applications of atomic force microscopy. Am. J. Physiol. 266, C1–C21.
Weisenhorn, A. L., Khorsandi, M., Kasas, S., Gotzos, V., and Butt, H. J. (1993) Deformation and height anomaly of soft surfaces studied with an AFM. Nanotech. 4, 106–113.
Hoh, J. H. and Schoenenberger, C. A. (1994) Surface morphology and mechanical properties of MDCK monolayers by atomic force microscopy. J. Cell Sci. 107, 1105–1114.
Shroff, S. G., Saner, D. R., and Lal, R. (1995) Dynamic micromechanical properties of cultured rat atrial myocytes measured by atomic force microscopy. Am. J. Physiol. 269, C286–C292.
Ricci, D., Tedesco, M., and Grattarola, M. (1997) Mechanical and morphological properties of living 3T6 cells probed via scanning force microscopy. Microsc. Res. Tech. 36, 165–171.
Sasaki, S., Morimoto, M., Haga, H., Kawabata, K., Ito, E., Ushiki, T., et al. (1998) Elastic properties of living fibroblasts as imaged using force modulation mode in atomic force microscopy. Arch. Histol. Cytol. 61, 57–63.
Sato, M., Nagayama, K., Kataoka, N., Sasaki, M., and Hane, K. (2000) Local mechanical properties measured by atomic force microscopy for cultured bovine endothelial cells exposed to shear stress. J. Biomech. 33, 127–135.
Mathur, A. B., Truskey, G. A., and Reichert, W. M. (2000) Atomic force and total internal reflection fluorescence microscopy for the study of force transmission in endothelial cells. Biophys. J. 78, 1725–1735.
Ookawa, K., Sato, M., and Ohshima, N. (1993) Morphological changes of endothelial cells after exposure to fluid-imposed shear stress: Differential responses induced by extracellular matrices. Biorheology. 30, 131–140.
Miyazaki, H. and Hayashi, K. (1999) Atomic force microscopic measurement of the mechanical properties of intact endothelial cells in fresh arteries. Med. Biol. Eng. Comput. 37, 530–536.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2004 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Miyazaki, H., Hayashi, K. (2004). Measurement of Mechanical Properties of Intact Endothelial Cells in Fresh Arteries. In: Braga, P.C., Ricci, D. (eds) Atomic Force Microscopy. Methods in Molecular Biology™, vol 242. Humana Press. https://doi.org/10.1385/1-59259-647-9:307
Download citation
DOI: https://doi.org/10.1385/1-59259-647-9:307
Publisher Name: Humana Press
Print ISBN: 978-1-58829-094-6
Online ISBN: 978-1-59259-647-8
eBook Packages: Springer Protocols