Methods for Detecting Cells in S Phase

  • Wei-Hsin Sun
  • Melvin L. DePamphilis
Part of the Methods in Molecular Biology™ book series (MIMB, volume 241)


S phase is that period of time in the cell-division cycle during which nuclear chromosomal deoxyribonucleic acid (DNA) is replicated (1,2). The time required for S phase depends on the size of the genome, the organism, and its developmental state. DNA replication requires only 15 to 20 min in budding yeast, but 6 to 7 h in mammalian cells. In organisms, such as frogs, fish, echinoderms, and flies that undergo rapid cell cleavage events at the beginning of their development, S phase takes only a few minutes during these initial cell cleavage events, but it takes several hours in late-stage embryos, adult animals, or cells cultured in vitro.


Replication Fork Glass Microscope Slide Wash Cell Giemsa Stain Transport Buffer 
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Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  • Wei-Hsin Sun
    • 1
  • Melvin L. DePamphilis
    • 2
  1. 1.National Institute of Mental HealthNational Institutes of HealthBethesda
  2. 2.National Institute of Child Health and Human DevelopmentNational Institutes of HealthBethesda

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