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2-D Electrophoresis of Plant Proteins

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2-D Proteome Analysis Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 112))

Abstract

The isolation of proteins from plants for two-dimentional electrophoresis (2-DE) is complicated by the plants’ hard cell walls, high frequency of modified N-termini, and insolubility of membrane proteins. Some of these difficulties are not limited to plant proteins, but are also rather common for proteins from other organisms.

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References

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© 1999 Humana Press Inc., Totowa, NJ

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Tsugita, A., Kamo, M. (1999). 2-D Electrophoresis of Plant Proteins. In: Link, A.J. (eds) 2-D Proteome Analysis Protocols. Methods in Molecular Biology, vol 112. Humana Press. https://doi.org/10.1385/1-59259-584-7:95

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  • DOI: https://doi.org/10.1385/1-59259-584-7:95

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-524-9

  • Online ISBN: 978-1-59259-584-6

  • eBook Packages: Springer Protocols

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