Abstract
Escherichia coli has been studied for many years by two-dimensional polyacrylamide gel electrophoresis (2-D gels) (1,2). This method has provided much information about the physiology of E. coli, particularly related to how the levels or synthesis rates of large numbers of proteins varied under different conditions.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
O’Farrell, P. H. (1975) High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250, 4007–4021.
VanBogelen, R. A., Abshire, K. Z., Pertsemlidis, A., Clark, R. L., and Neidhardt, F. C. (1996) Gene-protein database of Escherichia coli K-12, in Escherichia coli and Salmonella: Cellular and Molecular Biology, 6th ed. (Neidhardt, F. C, Curtiss, R., Gross, C, Ingraham, J. L., Lin, E. C. C, Low, K. B., et al., eds.), ASM, Washington, DC, pp. 2067–2117.
VanBogelen, R. A. and Olson, E. R. (1995) Application of 2-D protein gels in biotechnology. Biotechnol. Ann. Rev. 1, 69–103.
VanBogelen, R. A., Hutton, M. E., and Neidhardt, F. C. (1990) Gene-protein database of Escherichia coli K-12: Edition 3. Electrophoresis 11, 1131–1166.
Neidhardt, F. C, Bloch, P. L., and Smith, D. F. (1974) Culture medium for enterobacteria. J. Bacteriol. 119, 736–747.
Wanner, B. L., Kodaira, R., and Neidhardt, F. C. (1977) Physiological regulation of a decontrolled lac operon. J. Bacteriol. 130, 212–222.
VanBogelen, R. A. and Neidhardt, F. C. (1991) Gene-protein database of Escherichia coli K—12: Edition 4. Electrophoresis 12, 955–994.
Smith, M. W. and Neidhardt, F. C. (1983) Proteins induced by anaerobiosis in Escherichia coli. J. Bacteriol. 154, 336–343.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
VanBogelen, R.A., Neidhardt, F.C. (1999). Preparation of Escherichia coli Samples for 2-D Gel Analysis. In: Link, A.J. (eds) 2-D Proteome Analysis Protocols. Methods in Molecular Biology, vol 112. Humana Press. https://doi.org/10.1385/1-59259-584-7:21
Download citation
DOI: https://doi.org/10.1385/1-59259-584-7:21
Publisher Name: Humana Press
Print ISBN: 978-0-89603-524-9
Online ISBN: 978-1-59259-584-6
eBook Packages: Springer Protocols