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Preparation of Escherichia coli Samples for 2-D Gel Analysis

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2-D Proteome Analysis Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 112))

Abstract

Escherichia coli has been studied for many years by two-dimensional polyacrylamide gel electrophoresis (2-D gels) (1,2). This method has provided much information about the physiology of E. coli, particularly related to how the levels or synthesis rates of large numbers of proteins varied under different conditions.

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References

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© 1999 Humana Press Inc., Totowa, NJ

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VanBogelen, R.A., Neidhardt, F.C. (1999). Preparation of Escherichia coli Samples for 2-D Gel Analysis. In: Link, A.J. (eds) 2-D Proteome Analysis Protocols. Methods in Molecular Biology, vol 112. Humana Press. https://doi.org/10.1385/1-59259-584-7:21

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  • DOI: https://doi.org/10.1385/1-59259-584-7:21

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-524-9

  • Online ISBN: 978-1-59259-584-6

  • eBook Packages: Springer Protocols

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