Double-Label Analysis

  • Kelvin H. Lee
  • Michael G. Harrington
Part of the Methods in Molecular Biology book series (MIMB, volume 112)

Abstract

The detection of radiolabeled proteins is of fundamental importance in experimental biology. 35S- and 14C-labeled proteins can provide investigators with information about protein expression, synthesis, and degradation. Moreover, posttranslational modifications, such as phosphorylation, can be studied with the use of 32P. Traditional methods for detecting radiolabeled proteins involve somewhat lengthy exposures to photographic film. Quantitation from film-based autoradiography can be achieved by densitometry of repeated and various exposures to films because of the small linear dynamic range of photographic film.

Keywords

Electrophoresis Polyacrylamide Methionine TEMED 

References

  1. 1.
    Harrington, M. G., Hood, L. E., and Puckett, C. (1991) Simultaneous analysis of phosphoproteins and total cellular protein from PC12 cells. Methods: A Companion to Methods Enzymol. 3, 135–141.CrossRefGoogle Scholar
  2. 2.
    Johnston, R. J., Pickett, S. C., and Barker, D. L. (1990) Autoradiography using storage phosphor technology. Electrophoresis 11, 355–360.PubMedCrossRefGoogle Scholar
  3. 3.
    Johnston, R. J., Pickett, S. C., and Barker, D. L. (1991) Double-label image analysis using storage phosphor technology. Methods: A Companion to Methods Enzymol. 3, 128–134.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1999

Authors and Affiliations

  • Kelvin H. Lee
    • 1
  • Michael G. Harrington
    • 2
  1. 1.Department of Chemical EngineeringCornell UniversityIthaca
  2. 2.Department of BiologyCalifornia Institute of TechnologyPasadena

Personalised recommendations