2-D Electrophoresis Using Carrier Ampholytes in the First Dimension (IEF)

  • Mary F. Lopez
Part of the Methods in Molecular Biology book series (MIMB, volume 112)


Two-dimensional electrophoresis (2-DE) is potentially the most powerful technique known for resolving complex protein mixtures. Proteins can be purified to homogeneity in one step in most cases. The applications for 2-DE are numerous and diverse. They include the analysis of protein patterns in various organisms and tissues, monitoring of the purity of fractions derived from chro-matography (or other separation methods), and the determination of the characteristics of a particular protein, such as isoelectric point, molecular weight of subunits, and number of isoforms.


Casting System Carrier Ampholyte Complex Protein Mixture Anode Solution Acrylamide Solution 
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Copyright information

© Humana Press Inc., Totowa, NJ 1999

Authors and Affiliations

  • Mary F. Lopez
    • 1
  1. 1.ESA, Inc.Chelmsford

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