Abstract
Agrobacterium-mediated transformation of plants is now applicable to many dicotyledonous and also several monocotyledonous plant species. It can be used to transform many different species based on various factors: the broad host range of Agrobacterium (1), the regeneration responsiveness of many different explant tissues (2), and the utility of a wide range of selectable marker genes (3). In addition to tobacco, one of the first species that was routinely transformed using this method was Petunia hybrida.
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References
Richie, S W. and Hodeges, T. K. (1993) Cell culture and regeneration of transgenic plants, in Transgenic Plants, vol 1 (Kung, S. and Wu, R., eds.), Academic, San Diego, pp. 147–178
Jenes, B., Morre, H, Cao, J., Zhang, W. and Wu, R (1993). Techniques for gene transfer, in Transgenic Plants, vol 1 (Kung, S and Wu, R., eds), Academic, San Diego, pp. 125–146
Bowen, B. A. (1993) Markers for gene transfer, in Transgenic Plants, vol 1 (Kung, S. and Wu, R., eds.), Academic, San Diego, pp. 89–124
Gerats, A G M, Huits, H, Vrijlandt, E, Maraña, C, Souer, E, and Beld, M (1990) Molecular characterization of a nonautonomous transposable element (dTphl) of petunia Plant Cell 2, 1121–1128
Horsch, R B, Fry, J E., Hoffman, N. L., Eichholtz, D, Rogers, S C, and Fraley, R. T (1985) A simple and general method for transferring genes into plants Science 111, 1229–1231
Hooykaas, P J. J. (1989) Transformation of plant cells via Agrobacterium. Plant Mol Biol 13, 327–336
Bevan, M. (1984) Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res 12, 8711–8721
Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 15, 473–497.
Maniatis, T, Fritsch, E. F, and Sambrook, J (1982) Molecular cloning A Laboratory Manual Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
Dellaporte, S. L., Wood, J., and Hicks, J. B. (1983) A plant DNA minipreparation: version II. Plant Mol. Biol Rep. 1, 19–21
Wang, H., Qi, M, and Cutler, A J (1993) A simple method of preparing plant samples for PCR. Nucleic Acids Res 21, 4153–4154
Hoekema, A., Hirsch, P. R, Hooykaas, P J J., and Schilperoort, R. A (1983) A binary plant vector strategy based on separation of vir and T region of the Agrobacterwm tumefaciens Ti-plasmid Nature 303, 179–180
Lazo, G R., Stein, P. A, and Ludwig, R. A. (1991) A DNA transformation-competent Arabidopsis genomic library in Agrobactenum Bio/Technology 9, 963–967
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© 1999 Humana Press Inc., Totowa, NJ
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van der Meer, I.M. (1999). Agrobacterium-Mediated Transformation of Petunia Leaf Disks. In: Hall, R.D. (eds) Plant Cell Culture Protocols. Methods In Molecular Biology™, vol 111. Humana Press. https://doi.org/10.1385/1-59259-583-9:327
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DOI: https://doi.org/10.1385/1-59259-583-9:327
Publisher Name: Humana Press
Print ISBN: 978-0-89603-549-2
Online ISBN: 978-1-59259-583-9
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