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Plant Cell Culture Protocols pp 211–226Cite as

Production of Cybrids in Rapeseed (Brassica napus)

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Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 111))

Abstract

Protoplast fusion produces cells that contain a mixture of the DNA-containing organelles (nuclei, chloroplasts, and mitochondria) from both fusion parents. This chapter describes the production of rapeseed (Brassica napus) fusion-product cells where one of the nuclei is eliminated. In the absence of selection pressure, the remaining chloroplast/mitochondrial mixture segregates randomly as the cell divides, eventually creating different nuclear/chloroplast/mitochondrial combinations. These novel nucleo-cytoplasmic combinations are called cytoplasmic hybrids or “cybrids.”

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Notes

  1. 1.

    †

    Abbreviations: 2,4-D- 2,4-dichlorophenoxyacetic acid; IAA- 3-indoleacetic acid; GA3-gib-berellic acid

  2. 2.

    †

    Abbreviations: 2,4-D- 2,4-dichlorophenoxyacetic acid; IAA- 3-indoleacetic acid; GA3-gib-berellic acid

  3. 3.

    †

    Abbreviations: 2,4-D- 2,4-dichlorophenoxyacetic acid; IAA- 3-indoleacetic acid; GA3-gib-berellic acid

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Author information

Authors and Affiliations

  1. Biotechnology Strategies and Coordination Office, Agriculture and Agri-Food Canada, Nepean, Ontario, Canada

    Stephen Yarrow

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  1. Stephen Yarrow
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Editor information

Editors and Affiliations

  1. CPRO-DLO, Wageningen, The Netherlands

    Robert D. Hall

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© 1999 Humana Press Inc., Totowa, NJ

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Yarrow, S. (1999). Production of Cybrids in Rapeseed (Brassica napus). In: Hall, R.D. (eds) Plant Cell Culture Protocols. Methods In Molecular Biology™, vol 111. Humana Press. https://doi.org/10.1385/1-59259-583-9:211

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  • DOI: https://doi.org/10.1385/1-59259-583-9:211

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-549-2

  • Online ISBN: 978-1-59259-583-9

  • eBook Packages: Springer Protocols

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