Abstract
Methods for clonal propagation of the two major morphological groups of orchids, i.e., sympodials and monopodials, are presented. The first group, sympodials, includes such genera as Cymbidium, Cattleya, Dendrobium, and Oncidium. They are characterized by a multibranching rhizome that can supply an abundance of axillary shoots for use as explants. They were among the first orchids to be successfully propagated, and techniques for their in vitro initiation (i.e., establishment) and subsequent proliferation are well established (1–8). The second group, monopodials, include Phalaenopsis and Vanda, and are characterized by a single, unbranched axis that possesses few available axillary shoots for use as explants. Significantly different in their morphologies, these two groups require different approaches to explant selection and subsequent culturing. The successful large-scale micropropagation of monopodials is, in fact, a relatively recent achievement (9) and is the culmination of a wide variety of studies using different media compositions and supplements (10–20).
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References
Churchill, M. E (1973) Tissue culture of orchids New Phytol. 72, 161–166
Lindermann, E G P. (1970) Menstem culture of Cattleya Am. Orchid Soc Bull 39, 1002–1004.
Morel, G. (1960) Producing virus free Cymbidiums Am Orchid Soc Bull 29, 495–497
Morel, G (1964) A new means of clonal propagation of orchids Am Orchid Soc Bull 33, 473–478
Sagawa, Y (1966) Clonal propagation of Cymbidiums through shoot menstem culture. Am Orchid Soc Bull 35, 188–192
Mujib, A and Jana, B. K. (1994) Clonal propagation of Dendrobium madame pompadour through apical menstem. Adv Plant Sci 7, 340–346
Scully, R (1967) Aspects of menstem culture in the Cattleya alliance Am Orchid Soc Bull 36, 103–108
Wimber, D. (1963) Clonal multiplication of Cymbidiums through tissue culture of the shoot menstem Am Orchid Soc Bull. 32, 105–107.
Kuhn, L (1982) Orchid propagation in the EYMC Marie Selby Bot Gard Bull 9, 32–34
Gnesbach, R. J. (1983) The use of indoleacetylamino acids in the in vitro propagation of Phalaenopsis orchids Sci Hort 19, 363–366.
Intuwong, O. (1974) Clonal propagation of Phalaenopsis by shoot tip culture. Am Orchid Soc Bull 43, 893–895
Huang, L C (1984) Alternative media and method for Cattleya propagation by tissue culture. Am Orchid Soc Bull 53, 167–170
Rotor, G. (1949) a method of vegetative propagation of Phalaenopsis species and hybrids Am Orchid Soc Bull 18, 738–739
Sagawa, Y (1961) Vegetative propagation of Phalaenopsis stem cuttings. Am Orchid Soc Bull 30, 803–809
Scully, R (1966) Stem propagation of Phalaenopsis Am Orchid Soc Bull 35, 40–42
Lay, M and Fan, F. (1978) Studies on the tissue culture of orchids Orchid Rev Oct, 308–310
Tanaka, M. (1977) Clonal propagation of Phalaenopsis by leaf tissue culture. Am Orchid Soc Bull 46, 733–737
Zimmer, K and Pieper, W (1978) Clonal propagation of Phalaenopsis by excised buds. Orchid Rev July, 223–227
Ernst, R (1994) Effects of thidiazuron on in vitro propagation of Phalaenopsis and Doritaenopsis (Orchidaceae). Plant Cell Tissue Organ Cult. 39, 273–275
Tokuhara, K and Mn, M (1993) Micropropagation of Phalaenopsis and Doritaenopsis by cultunng shoot tips of flower stalk buds Plant Cell Re 13, 7–11
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© 1999 Humana Press Inc., Totowa, NJ
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Tisserat, B., Jones, D. (1999). Clonal Propagation of Orchids. In: Hall, R.D. (eds) Plant Cell Culture Protocols. Methods In Molecular Biology™, vol 111. Humana Press. https://doi.org/10.1385/1-59259-583-9:127
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DOI: https://doi.org/10.1385/1-59259-583-9:127
Publisher Name: Humana Press
Print ISBN: 978-0-89603-549-2
Online ISBN: 978-1-59259-583-9
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