Production of Cybrids in Rapeseed (Brassica napus)

  • Stephen Yarrow
Part of the Methods In Molecular Biology™ book series (MIMB, volume 111)

Abstract

Protoplast fusion produces cells that contain a mixture of the DNA-containing organelles (nuclei, chloroplasts, and mitochondria) from both fusion parents. This chapter describes the production of rapeseed (Brassica napus) fusion-product cells where one of the nuclei is eliminated. In the absence of selection pressure, the remaining chloroplast/mitochondrial mixture segregates randomly as the cell divides, eventually creating different nuclear/chloroplast/mitochondrial combinations. These novel nucleo-cytoplasmic combinations are called cytoplasmic hybrids or “cybrids.”

Keywords

Sucrose Recombination Agarose Polyethylene Glycol 

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Copyright information

© Humana Press Inc., Totowa, NJ 1999

Authors and Affiliations

  • Stephen Yarrow
    • 1
  1. 1.Biotechnology Strategies and Coordination OfficeAgriculture and Agri-Food CanadaNepeanCanada

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