Abstract
In human plasma, cholesteryl esters and triglycerides can exchange between various lipoprotein fractions through the action of one specific lipid transfer protein: the cholesteryl ester transfer protein (CETP) (1, 2). Since in vivo CETP activity results in the redistribution of neutral lipid species between pro-and antiatherogenic lipoprotein particles, a marked interest has been given to the evaluation of CETP mass and activity in human plasma; and during the last decade, several specific assays—including net mass transfer assays, isotopic transfer assays, and immunoassays—have been proposed. In fact, the cholesteryl ester transfer reaction is a complex process that integrates a sequence of events and is dependent on a number of factors, among them the CETP mass concentration, as well as the amount and the composition of the plasma lipoprotein substrates (2). Thus, the evaluation of plasma CETP activity should ideally integrate several methods in order to obtain a clear picture of what is occurring. Two distinct methods are described in this chapter: (1) a competitive enzyme-linked immunosorbent assay (ELISA) of the CETP protein; and (2) an isotopic transfer assay, which evaluates the activity of CETP as modulated by endogenous plasma factors.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Tall, A. R. (1995) Plasma lipid transfer proteins Annu Rev Biochem 64, 235–257.
Lagrost, L. (1994) Regulation of cholesteryl ester transfer protein (CETP) activity Review of in vitro and in vivo studies. Biochim Biophys Acta 1215, 209–236
Marcel, Y L, McPherson, R, Hogue, M, Czarnecka, H, Zawadzki, Z, Weech, P. K., Whitlock, M E, Tall, A R., and Milne, R W (1990) Distribution and concentration of cholesteryl ester transfer protein in plasma of normolipidemic subjects. J Clin. Invest 85, 10–17
Mezdour, H, Kora, I, Parra, H J, Tartar, A., Marcel, Y. L., and Fruchart, J C (1994) Two-site enzyme immunoassay of cholesteryl ester transfer protein with monoclonal and ohgoclonal antibodies Clin Chem 40, 593–597
Kukasawa, M, Arai, H, and Inoue, K (1992) Establishment of anti-human cholesteryl ester transfer protein monoclonal antibodies and radioimmunoassaying of the level of cholesteryl ester transfer protein in human plasma. J Biochem 111, 996–998.
Ritsch, A., Auer, B., Foger, B., Schwarz, S., and Patsch, J R (1993) Polyclonal antibody-based immunoradiometric assay for quantification of cholesteryl ester transfer protein J Lipid Res 34, 673–679.
Guyard-Dangremont, V, Lagrost, L, Gambert, P, and Lallemant, C. (1994) Competitive enzyme-linked immunosorbent assay of the human cholesteryl ester transfer protein (CETP). Chn Chim. Ada 231, 147–160
Son, Y S and Zilversmit, D B (1986) Increased hpid transfer activities in hyperhpidemic rabbit plasma Artenoscler 6, 345–351
Van Tol, A (1993) CETP-catalysed transfer of cholesteryl esters from HDL to apo B-containing hpoproteins in plasma from diabetic patients. Eur. J Clin Invest 23, 856
Swenson, T. L., Hesler, C. B, Brown, M L., Quinet, E, Trotta, P P., Haslanger, M. F, Gaeta, F C. A., Marcel, Y L, Milne, R W., and Tall, R (1989) Mechanism of cholesteryl ester transfer protein inhibition by a neutralizing monoclonal antibody and mapping of the monoclonal antibody epitope. J Biol Chem 264, 14,318–14,326
Lagrost, L. and Barter, P J. (1991) Effects of various non-esterified fatty acids on the particle size redistribution of high density hpoproteins induced by the human cholesteryl ester transfer protein. Biochim. Biophys Acta 1082, 204–210
Engvall, E (1980) Enzyme immunoassay ELISA and EMIT Methods Enzymol 70, 419–439.
Morton, R. E and Zilversmit, D B (1981) A plasma inhibitor of triglyceride and cholesteryl ester transfer activities J Biol. Chem. 256, 11,992–11,995.
Groener, J. E. M., Pelton, R. W, and Kostner, G. M. (1986) Improved estimation of cholesteryl ester transfer/exchange activity in serum or plasma. Clin Chem 32, 283–286.
Sparks, D L, Frohlich, J, Cullis, P., and Pritchard, P. H. (1987) Cholesteryl ester transfer activity in plasma measured by using solid phase-bound high-density lipoproteins Clin Chem 33, 390–393.
Florentin, E, Lagrost, L, Lallemant, C, and Gambert, P (1994) Polyacrylamide gradient gel electrophoresis as a method to measure transfers of radiolabeled cholesteryl esters between several plasma lipoprotein fractions. Anal Biochem 216, 352–357
Lagrost, L., Loreau, N, Gambert, P., and Lallemant, C. (1995) Immunospecific scintillation proximity assay of cholesteryl ester transfer protein activity Clin Chem 41, 914–919
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Lagrost, L. (1998). Determination of the Mass Concentration and the Activity of the Plasma Cholesteryl Ester Transfer Protein (CETP). In: Ordovas, J.M. (eds) Lipoprotein Protocols. Methods in Molecular Biology™, vol 110. Humana Press. https://doi.org/10.1385/1-59259-582-0:231
Download citation
DOI: https://doi.org/10.1385/1-59259-582-0:231
Publisher Name: Humana Press
Print ISBN: 978-0-89603-420-4
Online ISBN: 978-1-59259-582-2
eBook Packages: Springer Protocols