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Lipoprotein Protocols pp 153–166Cite as

Measurement of Lipoprotein Particles

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 110))

Abstract

Lipoproteins have been traditionally classified based on their hydrated densities or electrophoretic mobilities. In the 1960s Alaupovic (1) proposed that plasma lipoproteins could be viewed as a system of particles characterized by their apolipoprotein composition. This concept evolved in the proposal of using “apolipoprotem profiles” for typing dyslipoproteinemias. Along these lines, several recent reports have shown that certain apolipoprotein profiles may be better predictors of lipoprotein disorders than the traditional lipid profiles (2). During the last decade, immunological methods have taken advantage of the specificity provided by polyclonal or monoclonal antibodies (PAbs, MAbs) to identify, separate, and measure specific lipoprotein particles directly in plasma (3). The application of these techniques to clinical and epidemiological studies has shown that these apolipoprotein-specific subclasses may be important diagnostic tools for dyslipoproteinemia, as well as useful predictors for coronary-artery disease (2, 4–6). Moreover, they may provide useful clues about the chemical nature of the corresponding lipoprotein particles

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Author information

Authors and Affiliations

  1. Service de Recherche sur les Lipoprotéines et L’ Athérosclérose (Serlia) et Inserm, Institut Pasteur de Lille, France

    Catherine Fievet & Jean-Charles Fruchart

Authors
  1. Catherine Fievet
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  2. Jean-Charles Fruchart
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Editors and Affiliations

  1. Tufts University, Boston, MA

    Jose M. Ordovas

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© 1998 Humana Press Inc., Totowa, NJ

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Fievet, C., Fruchart, JC. (1998). Measurement of Lipoprotein Particles. In: Ordovas, J.M. (eds) Lipoprotein Protocols. Methods in Molecular Biology™, vol 110. Humana Press. https://doi.org/10.1385/1-59259-582-0:153

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  • DOI: https://doi.org/10.1385/1-59259-582-0:153

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-420-4

  • Online ISBN: 978-1-59259-582-2

  • eBook Packages: Springer Protocols

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