Preparative Isolation of Plasma Lipoproteins Using Fast Protein Liquid Chromatography (FPLC)

  • Jose M. Ordovas
  • Doreen Osgood
Part of the Methods in Molecular Biology™ book series (MIMB, volume 110)


The presence of lipids wtthin the hpoprotein particles confers these complexes with a lower density compared with other serum proteins. Based on thts property, lipoproteins were classically separated into four major classes desrgnated as chylomicrons, very low-denstty lipoproteins (VLDL), low-denstty lipoprotems (LDL), and high-density lipoproteins (HDL). In addition to then densities, these macromolecules differ from other plasma proteins because of their size (see Table 1). This characteristic makes these particles suitable to be separated using size-exclusion chromatography (1).


Lipoprotein Fraction Fraction Collector Fast Protein Liquid Chromatography Agarose Column Cholesterol Measurement 
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Copyright information

© Humana Press Inc., Totowa, NJ 1998

Authors and Affiliations

  • Jose M. Ordovas
    • 1
  • Doreen Osgood
    • 1
  1. 1.Lipid Metabolism LaboratoryJM-USDA-Human Nutrition Research Center on Aging at Tufts UniversityBoston

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