Abstract
Lipoprotein lipase (LPL) plays a central role in lipoprotein metabolism. LPL is the major enzyme responsible for the hydrolysis of triglycerides in VLDL and chylomicrons. Following hydrolysis of the triglyceride core of lipoproteins, some of the LPL molecules remain associated with chylomicron remnants and intermediate density lipoproteins (IDL) derived from VLDL. There is now accumulating evidence that these lipase molecules may play a role in the removal of chylomicron remnants and IDL by the liver and other tissues (1–4). This second function of LPL is independent of its catalytic activity.
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References
Enerback, S. and Gimble, J. M. (1993) Lipoprotein lipase gene expression: physiological regulators at the transcriptional and post-translational level. Biochim. Biophys. Ada. 1169, 107–125.
Olivecrona, T. and Bengtsson-Olivecrona, G. (1993) Lipoprotein lipase and hepatic lipase. Curr. Opin. Lipidol. 4, 187–196.
Santamarina-Fojo, S. and Dugi, K. A. (1994) Structure, function and role of lipoprotein lipase in lipoprotein metabolism. Curr. Opin. Lipidol. 5, 117–125.
Goldberg, I. J. (1996) Lipoprotein lipase and lipolysis: central roles in lipoprotein metabolism and atherogenesis. J. Lipid Res. 37, 693–707.
Cheung, A. H., Bensadoun, A., and Cheng, C.-F. (1979) Direct solid phase radio-immunoassay for chicken lipoprotein lipase. Anal. Biochem. 94, 346–357.
Bensadoun, A., Ehnholm, C., Steinberg, D., and Brown, W. V. (1974) Purification and characterization of lipoprotein lipase from pig adipose tissue. J. Biol. Chem. 249, 2220–2227.
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© 1999 Humana Press Inc, Totowa, NJ
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Bensadoun, A., Hsu, J., Hughes, B. (1999). Large-Scale Lipoprotein Lipase Purification from Adipose Tissue. In: Doolittle, M., Reue, K. (eds) Lipase and Phospholipase Protocols. Methods in Molecular Biology™, vol 109. Humana Press. https://doi.org/10.1385/1-59259-581-2:145
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DOI: https://doi.org/10.1385/1-59259-581-2:145
Publisher Name: Humana Press
Print ISBN: 978-0-89603-546-1
Online ISBN: 978-1-59259-581-5
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