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Purification of Rat Hepatic Lipase Essentially Free of Apolipoprotein E and Apolipoprotein B

  • André Bensadoun
  • Barry Hughes
  • Kristan Melford
  • Jean Hsu
  • Dawn L. Braesaemle
Part of the Methods in Molecular Biology™ book series (MIMB, volume 109)

Abstract

Hepatic lipase (HL) is necessary for efficient clearance of triglyceride-rich lipoproteins from plasma (1). In humans, HL deficiency leads to the accumulation of triglyceride-rich intermediate density lipoproteins (IDL), LDL, and large HDL (2, 3, 4). Inhibition of hepatic lipase in rats with specific antibodies to HL (5, 6, 7, 8, 9, 10, 11, 12) causes similar changes in plasma lipoprotein patterns, with accumulation of IDL and chylomicron remnants in the low density ranges and increases in the large HDL subfractions. In isolated, perfused rat livers inhibition of hepatic lipase with antibodies or removal of HL by heparin perfusion reduces the uptake of chylomicron remnants (13). Finally, recent studies with cultured hepatoma cells over-expressing human HL (14) demonstrate a threefold increase in binding and uptake of β-VLDL and chylomicron remnants when compared to non-transfected cells. Hence, several studies have shown that decreased HL activity inhibits clearance of triglyceride-rich lipoproteins, while overexpression of HL facilitates clearance of these particles.

Keywords

Liver Perfusate Hepatic Lipase Intermediate Density Lipoprotein Chylomicron Remnant Hepatic Lipase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Jansen, H. and Hülmann, W. C. (1985) Enzymology and physiological role of hepatic lipase. Biochem. Soc. Trans. 13, 24–26.PubMedGoogle Scholar
  2. 2.
    Hegele, R. A., Vezina, C., Moorjani, S., Lupien, P. J., Gagne, C., Brun, L. D., Little, J. A., and Connelly, P. W., (1991) A hepatic lipase gene mutation associated with heritable lipolytic deficiency. J. Clin. Endocrinol. Metab. 72, 730–732.PubMedCrossRefGoogle Scholar
  3. 3.
    Connelly, P. W., Maguire, G. F., Lee, M. and Little, J. A. (1990) Plasma lipoproteins in familial hepatic lipase deficiency. Arteriosclerosis 10, 40–48PubMedGoogle Scholar
  4. 4.
    Hegele, R. A., Little, J. A., Vezina, C., Maguire, G. F., Tu, L., Wolever, T. S., Jenkins, D. J. A., and Connelly, P. W. (1993) Hepatic lipase deficiency. Clinical, biochemical, and molecular genetic characteristics. Arterioscler. Thromb. 13, 720–728.PubMedGoogle Scholar
  5. 5.
    Goldberg, I. J., Lee, N. A., Paterniti, J. R., Jr., Ginsberg, H. N., Lindgren, F. T., and Brown, W. V. (1992) Lipoprotein metabolism during acute inhibition of hepatic triglyceride lipase in the cynomologus monkey. J. Clin. Invest. 70, 1184–1192.CrossRefGoogle Scholar
  6. 6.
    Kuusi, T., Kinnunen, P. K. J., and Nikkila, E. A. (1979) Hepatic endothelial lipase antiserum influences rat plasma low and high density lipoproteins in vivo. FEBS Lett. 104, 384–388.PubMedCrossRefGoogle Scholar
  7. 7.
    Jansen, H., van Tol, A. and Hülsmann, W. C. (1980) On the metabolic function of heparin-releasable liver lipase. Biochem. Biophys. Res. Commun. 92, 53–59.PubMedCrossRefGoogle Scholar
  8. 8.
    Grosser, J., Schrecker, O., and Greten, H. (1981) Function of hepatic triglyceride lipase in lipoprotein metabolism. J. Lipid Res. 22, 437–442.PubMedGoogle Scholar
  9. 9.
    Daggy, B. P. and Bensadoun, A. (1986) Enrichment of apolipoprotein B-48 in the LDL density class following in vivo inhibition of hepatic lipase. Biochim. Biophys. Acta 877, 252–261.PubMedGoogle Scholar
  10. 10.
    Demacker, P. N. M., Hijmans, A. G. M., Stalenhoef, A. F. H., and van’t Laar (1988) Studies on the function of hepatic lipase in the cat after immunological blockade of the enzyme in vivo. Atherosclerosis 69, 173–183.PubMedCrossRefGoogle Scholar
  11. 11.
    Murase, T. and Itakura, H. (1981) Accumulation of intermediate density lipoprotein after intravenous administration of hepatic triglyceride lipase antibody in rats. Atherosclerosis 39, 293–300.PubMedCrossRefGoogle Scholar
  12. 12.
    Sultan, F., Lagrange, D., Jansen, H. and Griglio, S. (1990) Inhibition of hepatic lipase activity impairs chylomicron remnant removal in rats. Biochim. Biophys. Acta 1042, 150–152.PubMedGoogle Scholar
  13. 13.
    Shafi, S., Brady, S. E., Bensadoun, A. and Havel, R. J. (1994) Role of hepatic lipase in the uptake and processing of chylomicron remnants in rat liver. J. Lipid Res. 35, 709–720.PubMedGoogle Scholar
  14. 14.
    Ji, Z. S., Lauer, S. J., Fazio, S., Bensadoun, A., Taylor, J. M., and Mahley, R. W. (1994) Enhanced binding and uptake of remnant lipoproteins by hepatic lipase-secreting hepatoma cells in culture. J. Biol. Chem. 269, 23,838–23,844.Google Scholar
  15. 15.
    Umbreit, W. W., Burris, R. H., and Stauffer, J. F. (1957) Manometric Techniques, Burgess, pp. 149.Google Scholar
  16. 16.
    Cisar, L. A. and Bensadoun, A. (1985) Enzyme-linked immunosorbent assay for rat hepatic triglyceride lipase. J. Lipid Res. 26, 380–386.PubMedGoogle Scholar
  17. 17.
    Brasaemle, D., Cornely-Moss, K., and Bensadoun, A. (1993) Hepatic lipase treatment of chylomicron remnants increases exposure of apolipoprotein E. J. Lipid Res. 34, 455–465.PubMedGoogle Scholar

Copyright information

© Humana Press Inc, Totowa, NJ 1999

Authors and Affiliations

  • André Bensadoun
    • 1
  • Barry Hughes
    • 2
  • Kristan Melford
    • 2
  • Jean Hsu
    • 2
  • Dawn L. Braesaemle
    • 2
  1. 1.Department of Biochemistry, Division of Nutritional ScienceCornell UniversityIthaca
  2. 2.Department of BiochemistryCornell UniversityIthaca

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