Abstract
Chemokines are a family of structurally related, small chemoattractant proteins that interact with specific seven-transmembrane, G-protein-coupled receptors that mediate cell migration in chemotactic gradients (1,2). Chemokine-receptor expression is finely regulated during leukocyte differentiation, maturation, and activation, causing each cell subpopulation to express a specific set of chemokine receptors. Although they were initially considered as simple leukocyte chemoattractants, chemokines are now known to be implicated in diverse phases of the immune response, and their significance is becoming apparent in many areas of biomedicine, including cancer, human immunodeficiency virus (HIV)-1 infection, asthma, and cardiovascular disease (3). This has greatly increased interest in studying how chemokines exert their activities at the molecular level, which obviously requires specific reagents. Monoclonal antibodies (mAbs) that recognize chemokines or chemokine receptors are among the most useful tools for this purpose, particularly for cell expression and neutralization studies.
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Mackay, C. R. (2001) Chemokines: immunology’s high impact factors. Nature Immunol. 2, 95–101.
Zlotnik, A. and Yoshie, O. (2000) Chemokines: a new classification system and their role in immunity. Immunity 12, 121–127.
Gerard, C. and Rollins, B. J. (2001) Chemokines and disease. Nature Immunol. 2, 108–115.
Gausepohl, H., Boulin, C., Kraft, M., and Frank, R. W. (1992) Automated multiple peptide synthesis. Peptide Res. 5, 315–320.
Tijssen, P. (1985) Preparation of enzyme-antibody or other enzyme-macromolecule conjugates, in Laboratory Techniques in Biochemistry and Molecular Biology (Burdon, R. H. and van Knippenberg, P. H., eds.), Elsevier Science, New York, pp. 221–296.
Martin-Belmonte, F., Kremer, L., Albar, J. P., Marazuela, M., and Alonso, M. A. (1998) Expression of the MAL gene in the thyroid: the MAL proteolipid, a component of glycolipid-enriched membranes, is apically distributed in thyroid follicles. Endocrinology 139, 2077–2084.
Ausubel, F. M., Brent, R., Kingston, R. E., et al. (1989) Current Protocols in Molecular Biology, Green Publishing Associates, New York.
Harlow, E. and Lane, D. E. (1988) Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory, Plainview, NY.
Kremer, L., Carramolino, L., Goya, I., et al. (2001) The transient expression of C-C chemokine receptor 8 in thymus identifies a thymocyte subset committed to become CD4+ single-positive T cells. J. Immunol. 166, 218–225.
Didsbury, J. R., Uhing, R. J., Tomhave, E., Gerard, C., Gerard, N., and Snyderman, R. (1991) Receptor class desensitization of leukocyte chemoattractant receptors. Proc. Natl. Acad. Sci. USA 88, 11,564–11,568.
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© 2004 Humana Press Inc., Totowa, NJ
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Kremer, L., Márquez, G. (2004). Generation of Monoclonal Antibodies Against Chemokine Receptors. In: D’Ambrosio, D., Sinigaglia, F. (eds) Cell Migration in Inflammation and Immunity. Methods in Molecular Biology™, vol 239. Humana Press. https://doi.org/10.1385/1-59259-435-2:243
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DOI: https://doi.org/10.1385/1-59259-435-2:243
Publisher Name: Humana Press
Print ISBN: 978-1-58829-102-8
Online ISBN: 978-1-59259-435-1
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