Abstract
Leukocyte recruitment has been recognized as an early event in inflammatory processes since the late 19th century. Accumulation and trafficking of leukocytes in tissues under physiological and pathological conditions are orderly (typically neutrophils precede mononuclear cells) and selective because, in certain states, one or more leukocyte subsets are recruited preferentially (e.g., eosinophils in allergy). The current paradigm of recruitment is that of a multistep process involving the action of chemotactic signals (1,2).
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Butcher, E. C. (1991) Leukocyte-endothelial cell recognition: three (or more) steps to specificity and diversity. Cell 67, 1033–1036.
Mantovani, A., Bussolino, F., and Dejana, E. (1992) Cytokine regulation of endothelial cell function. FASEB J. 6, 2591–2599.
Ward, P. A., Remold, H. G., and David, J. R. (1970) The production by antigen-stimulated lymphocytes of a lekotactic factor distinct from migration inhibitory factor. Cell Immunol. 1, 162–174.
Meltzer, M. S., Stevenson, M. M., and Leonard, E. J. (1977) Characterization of macrophage chemotaxis in tumor cell cultures and comparison with lymphocyte-derived chemotactic factors. Cancer Res. 37, 721–725.
Bottazzi, B., Polentarutti, N., Acero, R., et al. (1983) Regulation of the macrophage content of neoplasms by chemoattractants. Science 220, 210–212.
Bottazzi, B., Polentarutti, N., Balsari, A., et al. (1983) Chemotactic activity for mononuclear phagocytes of culture supernatants from murine and human tumor cells: evidence for a role in the regulation of the macrophage content of neoplastic tissues. Int. J. Cancer 31, 55–63.
Bottazzi, B., Ghezzi, P., Taraboletti, G., et al. (1985) Tumor-derived chemotactic factor(s) from human ovarian carcinoma: evidence for a role in the regulation of macrophage content of neoplastic tissues. Int. J. Cancer 36, 167–173.
Mantovani, A., Bottazzi, B., Colotta, F., Sozzani, S., and Ruco, L. (1992) The origin and function of tumor-associated macrophages. Immunol. Today 13, 265–270.
Valente, A. J., Fowler, S. R., Sprague, E. A., Kelley, J. L., Suenram, C. A., and Schwartz, C. J. (1984) Initial characterization of a paripheral blood mononuclear cell chemoattractant derived from cultured arterial smooth muscle cells. Am. J. Pathol. 117, 409–417.
Zullo, J. N., Cochran, B. H., Huang, A. S., and Stiles, C. D. (1985) Platelet-derived growth factor and double-stranded ribonucleic acids stimulate expression of the same genes in 3T3 cells. Cell 43, 793–800.
Rollins, B. J., Morrison, E. D., and Stiles, C. D. (1988) Cloning and expression of JE, a gene inducible by platelet-derived growth factor and whose product has cytokine-like properties. Proc. Natl. Acad. Sci. USA 85, 3738–3742.
Yoshimura, T., Robinson, E. A., Tanaka, S., Appella, E., Kuratsu, J., and Leonard, E. J. (1989) Purification and amino acid analysis of two human glioma-derived monocyte chemoattractants. J. Exp. Med. 169, 1449–1459.
Matsushima, K., Larsen, C. G., DuBois, G. C., and Oppenheim, J. J. (1989) Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line. J. Exp. Med. 169, 1485–1490.
Zachariae, C. O., Anderson, A. O., Thompson, H. L., et al. (1990) Properties of monocyte chemotactic and activating factor (MCAF) purified from a human fibrosarcoma cell line. J. Exp. Med. 171, 2177–2182.
Van Damme, J., Decock, B., Lenaerts, J. P., et al. (1989) Identification by sequence analysis of chemotactic factors for monocytes produced by normal and transformed cells stimulated with virus, double-stranded RNA or cytokine. Eur. J. Immunol. 19, 2367–2373.
Graves, D. T., Jiang, Y. L., Williamson, M. J., and Valente, A. J. (1989) Identifcation of monocyte chemotactic activity produced by malignant cells. Science 245, 1490–1493.
Furutani, Y., Nomura, H., Notake, M., et al. (1989) Cloning and sequencing of the cDNA for human monocyte chemotactic and activating factor (MCAF). Biochem. Biophys. Res. Commun. 159, 248–255.
Yoshimura, T., Yuhki, N., Moore, S. K., Appella, E., Lerman, M. I., and Leonard, E. J. (1989) Human monocyte chemoattractant proein-1 (MCP-1). Full-length cDNA cloning, expression in mitogen-stimulation blood mononuclear meukocytes, and sequence similarity to mouse competence gene JE. FEBS Lett. 244, 487–493.
Bottazzi, B., Colotta, F., Sica, A., Nobili, N., and Mantovani, A. (1990) A chemoattractant expressed in human sarcoma cells (tumor-derived chemotactic factor, TDCF) is identical to monocyte chemoattractant protein-1/monocyte chemotactic and activating factor (MCP-1/MCAF). Int. J. Cancer 45, 795–797.
D’Amico, G., Bianchi, G., Bernasconi, S., et al. (1998) Adhesion, transendothelial migration, and reverse transmigration of in vitro cultured dendritic cells. Blood 92, 207–214.
Falk, W., Goodwin, R. H. Jr., and Leonard, E. J. (1980) A 48-well microchemotaxis assembly for rapid and accurate measurement of leukocyte migration. J. Immunol. Methods 33, 239–245.
Sozzani, S., Luini, W., Molino, M., et al. (1991) The signal transduction pathway involved in the migration induced by a monocyte chemotactic cytokine. J. Immunol. 147, 2215–2221.
Wilkinson, P. C. (1998) Assays of leukocyte locomotion and chemotaxis. J. Immunol. Methods 216, 139–153.
Romano, M., Sironi, M., Toniatti, C., et al. (1997) Role of IL-6 and its soluble receptor in induction of chemokines and leukocyte recruitment. Immunity 6, 315–325.
Springer, T. A. (1994) Traffic signal for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell 76, 301–314.
Adams, D. H. and Shaw, S. (1994) Leucocyte-endothelial interactions and regulation of leucocyte migration. Lancet 343, 831–836.
Bianchi, G., Sironi, M., Ghibaudi, E., et al. (1993) Migration of natural killer cells across endothelial cell monolayers. J. Immunol. 151, 5135–5144.
Allavena, P., Paganin, C., Martin Padura, I., et al. (1991) Molecules and structures involved in the adhesion of natural killer cells to vascular endothelium. J. Exp. Med. 173, 439–448.
Williams, R. L., Courtneidge, S. A., and Wagner, E. F. (1988) Embryonic lethalities and endothelial tumors in chimeric mice expressing polyoma virus middle T onco-gene. Cell 52, 121–131.
Bussolino, F., De Rossi, M., Sica, A., et al. (1991) Murine endothelioma cell lines transformed by polyoma middle T oncogene as target for and producers of cytokines. J. Immunol. 147, 2122–2129.
Garlanda, C., Parravicini, C., Sironi, M., et al. (1994) Progressive growth in immunodeficient mice and host cell recruitment by mouse endothelial cells transformed by polyoma middle-sized T antigen: implications for the pathogenesis of opportunistic vascular tumors. Proc. Natl. Acad. Sci. USA 91, 7291–7295.
Taraboletti, G., Belotti, D., Dejana, E., Mantovani, A., and Giavazzi, R. (1993) Endothelial cell migration and invasiveness are induced by a soluble factor produced by murine endothelioma cells transformed by polyoma virus middle T onco-gene. Cancer Res. 53, 3812–3816.
Bocchietto, E., Guglielmetti, A., Silvagno, F., et al. (1993) Proliferative and migratory responses of murine microvascular endothelial cells to granulocyte-colony-stimulating factor. J. Cell Physiol. 155, 89–95.
Vecchi, A., Garlanda, C., Lampugnani, M. G., et al. (1994) Monoclonal antibodies specific for endothelial cells of mouse blood vessels. Their application in the identification of adult and embryonic endothelium. Eur. J. Cell Biol. 63, 247–254.
Piali, L., Albelda, S. M., Baldwin, H. S., Hammel, P., Gisler, R. H., and Imhof, B. A. (1993) Murine platelet endothelial cell adhesion molecule (PECAM-1)/CD31 modulates beta 2 integrins on lymphokine-activated killer cells. Eur. J. Immunol. 23, 2464–2471.
Hahne, M., Jager, U., Isenmann, S., Hallmann, R., and Vestweber, D. (1993) Five tumor necrosis factor-inducible cell adhesion mechanisms on the surface of mouse endothelioma cells mediate the binding of leukocytes. J. Cell Biol. 121, 655–664.
Kriehuber, E., Breiteneder-Geleff, S., Groeger, M., et al. (2001) Isolation and characterization of dermal lymphatic and blood endothelial cells reveal stable and functionally specialized cell lineages. J. Exp. Med. 194, 797–808.
Mancardi, S., Stanta, G., Dusetti, N., et al. (1999) Lymphatic endothelial tumors induced by intraperitoneal injection of incomplete Freund’s adjuvant. Exp. Cell Res. 246, 368–375.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2004 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Sozzani, S., Vecchi, A., Allavena, P., Mantovani, A. (2004). Chemotaxis and Interaction with Vascular or Lymphatic Endothelium. In: D’Ambrosio, D., Sinigaglia, F. (eds) Cell Migration in Inflammation and Immunity. Methods in Molecular Biology™, vol 239. Humana Press. https://doi.org/10.1385/1-59259-435-2:1
Download citation
DOI: https://doi.org/10.1385/1-59259-435-2:1
Publisher Name: Humana Press
Print ISBN: 978-1-58829-102-8
Online ISBN: 978-1-59259-435-1
eBook Packages: Springer Protocols