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Real-Time Quantitative RT-PCR to Detect Fusion Gene Transcripts Associated With AML

  • Rajinder Flora
  • David Grimwade
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 91)

Abstract

Relapse remains a major cause of treatment failure in acute myeloid leukemia (AML); however, patients destined to relapse cannot be reliably distinguished on the basis of pretreatment characteristics. Hence, there has been considerable interest in strategies for detection of minimal residual disease (MRD) as a means of identifying patients at high risk from relapse who could benefit from additional therapy. Moreover, MRD monitoring also affords the opportunity to reveal subsets of patients at low risk of relapse who could be spared excessive therapy, thereby reducing the risk of treatment related mortality and morbidity. Previous studies have confirmed that MRD monitoring using PCR- or flow cytometric-based approaches provides key independent prognostic information in a number of subtypes of leukemia and is increasingly being used for risk stratification as a means of directing treatment approach (see refs. 1,2 for recent reviews).

Keywords

Acute Myeloid Leukemia Fusion Gene Minimal Residual Disease Laminar Flow Hood Plasmid Standard 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Rajinder Flora
    • 1
  • David Grimwade
    • 1
  1. 1.Division of Medical and Molecular GeneticsGuy’s, King’s, and St. Thomas’ School of MedicineLondonUK

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