Enzyme-Prodrug Systems

Thymidine Phosphorylase/5′-Deoxy-5-Fluorouridine
  • Alexandre Evrard
  • Joseph Ciccolini
  • Pierre Cuq
  • Jean-Paul Cano
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 90)

Abstract

Thymidine phosphorylase (E.C. 2.4.2.4) (TP), also described as the angiogenic platelet-derived endothelial cell growth factor (PD-ECGF) (1, 2, 3, 4), is a homodimeric enzyme with a monomeric molecular mass of about 55 kDa (5,6) that phosphorolytically cleaves thymidine to yield thymine and deoxyribose-1-phosphate (dR-1-P) (7,8). TP is expressed in various human cells and tissues and plays a role in plasma thymidine homeostasis (9, 10, 11). The levels of expression in different human tissues can vary up to 15-fold (12). Moreover, TP levels are increased in several types of malignant tumors when compared to the non-neoplastic regions of these tissues (13) and also in the plasma from tumor-bearing animals and cancer patients (14).

Keywords

Vortex Toxicity HPLC DMSO Syringe 

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Alexandre Evrard
    • 1
  • Joseph Ciccolini
    • 2
  • Pierre Cuq
    • 1
  • Jean-Paul Cano
    • 1
  1. 1.Department of Toxicology, Faculty of PharmacyUniversity of MontpellierMontpellierFrance
  2. 2.Department of Pharmacokinetics and Toxicokinetics, Faculty of PharmacyUniversity of Aix-MarseilleMarseilleFrance

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