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Methodologies for Processing Biodegradable and Natural Origin Scaffolds for Bone and Cartilage Tissue-Engineering Applications

  • Manuela E. Gomes
  • Patrícia B. Malafaya
  • Rui L. Reis
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 238)

Abstract

The ultimate goal of tissue engineering is to replace, repair or enhance the biological function of damaged, absent or dysfunctional elements of a tissue or an organ. Engineered tissues are produced by using cells that are manipulated through their extracellular environment to develop living biological substitutes for tissues that are lacking or malfunctioning (1, 2, 3, 4, 5). Many different strategies may be used to accomplish this goal. Among the most important factors that determine the selection of the best strategy for developing and utilizing engineered tissues are the technical feasibility, the required properties of the implant, and the interaction of the host with the graft.

Keywords

Tissue Engineering Injection Molding Biodegradable Polymer Compression Molding Fuse Deposition Modeling 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. 1.
    Hardin-Young, J., Teumer, J., Ross, R. N., and Parenteau, N. L. (2000) Approaches to transplanting 1 engineered cells and tissues, in Principles of Tissue Engineering, 2nd ed. (Lanza, R., Langer, R., Vacanti, J., eds.), Academic Press, New York, NY, pp. 281–291.CrossRefGoogle Scholar
  2. 2.
    Bruder, S. P. and Caplan, A. I. (1997) Bone regeneration thought cellular engineering, in Principles of Tissue Engineering (Lanza, R., Langer, R., and Chick, W., eds.), Academic Press, New York, NY, pp. 273–293.Google Scholar
  3. 3.
    Yang, S., Leong, K. F., Du, Z., and Chua, C. K. (2001) The design of scaffolds for use in tissue engineering. Part I. Traditional factors. Tissue Engineering 7, 679–689.CrossRefGoogle Scholar
  4. 4.
    Tabata, Y. (2001) Recent progress in tissue engineering. Research Focus 6, 483–487.Google Scholar
  5. 5.
    Freyman, T. M., Yannas, I. V., and Gibson, L. J. (2001) Cellular materials as porous scaffolds for tissue engineering. Progress in Materials Science 46, 273–282.CrossRefGoogle Scholar
  6. 6.
    Pachence, J. M. and Kohn, J. (1997) Biodegradable polymers for tissue engineering, in Principles of Tissue Engineering (Lanza, R., Langer, R., Chick, W., eds.), Academic Press, New York, NY, pp. 273–293.Google Scholar
  7. 7.
    Thomson, R. C., Wake, M. C., Yaszemski, M., and Mikos, A. G. (1995) Biodegradable polymer scaffolds to regenerate organs. Adv. Polym. Sci. 122, 247–274.Google Scholar
  8. 8.
    Agrawal, C. M., Athanasiou, K. A., and Heckman, J. D. (1997) Biodegradable PLA-PGA polymers for tissue engineering in orthopaedics. Materials Science Forum 250, 115–228.CrossRefGoogle Scholar
  9. 9.
    Thomson, R., Yaszemski, M., and Mikos, A. (1997) Polymer Scaffold processing, in Principles of Tissue Engineering (Lanza, R., Langer, R., and Chick, W., eds.), Academic Press, New York, NY, pp. 263–272.Google Scholar
  10. 10.
    Lu, L. and Mikos, A. (1996) The importance of new processing techniques in tissue engineering. MRS Bulletin 21, 28–32.Google Scholar
  11. 11.
    Mikos, A. G., Thorsen, A. J., Czerwonka, L. A., Bao, Y., and Langer, R. B. (1994) Preparation and characterization of poly(l-lactid acid) foams. Polymer 1068–1077.Google Scholar
  12. 12.
    Langer, R. (1999) Selected advances in drug delivery and tissue engineering. J. Control. Release 62, 7–11.CrossRefGoogle Scholar
  13. 13.
    Mikos, A. G., Sarakinos, G., Leite, S. M., Vacanti, J. P., and Langer, R. (1993) Laminated three-dimensional biodegradable foams for use in tissue engineering. Biomaterials 14, 323–330.CrossRefGoogle Scholar
  14. 14.
    Mikos, A. G., Bao, Y., Cima, L. G., Ingeber, D. E., Vacanti, J. P., and Langer, R. B. (1993) Preparation of poly(glycolic acid) bonded fiber structures for cell attachment and transplantation. J. Biomed. Mater. Res. 27, 183–189.CrossRefGoogle Scholar
  15. 15.
    Mooney, D. J., Baldwin, D. F., Suh, N. P., and Vacanti, J. P. (1996) Novel approach to fabricate porous sponges of poly (d,l-lactid-co-glycolic acid) without the use of organic solvents. Biomaterials 17, 1417–1422.CrossRefGoogle Scholar
  16. 16.
    Gomes, M. E., Ribeiro, A. S., Malafaya, P. B., Reis, R. L., and Cunha, A. M. (2001) A new approach based on injection moulding to produce biodegradable starch-based polymeric scaffolds: morphology, mechanical and degradation behaviour. Biomaterials 22, 883–889.CrossRefGoogle Scholar
  17. 17.
    Thompson, R. C., Yaszemski, M. J., and Powders, J. M. (1995) Fabrication of biodegradable polymer scaffolds to engineer trabecular bone. Journal Biomaterials Science—Polymer Edition 7, 23–28.CrossRefGoogle Scholar
  18. 18.
    Gomes, M. E., Reis, R. L., and Cunha, A. M. (2002) Alternative tissue engineering scaffolds based on starch: processing methodologies, morphology, degradation behaviour and mechanical Properties. Materials Science and Engineering: C Biomimetic and Supramolecular Systems 20, 19–26.Google Scholar
  19. 19.
    Gomes, M. E. (2002), Godinho, J. S., Reis, R. L., and Cunha, A. M. Design and processing of starch based scaffolds for hard tissue engineering. Journal of Applied Medical Polymers 6, 75–80.Google Scholar
  20. 20.
    Malafaya, P. B., Elvira, C., Gallardo, A., Román, J. S., and Reis, R. L. (2001) Porous starch-based drug delivery system processed by a microwave route. J. Biomater. Sci.—Polym. Ed. 12, 1227–1241.CrossRefGoogle Scholar
  21. 21.
    Hutmacher, D. W. (2000) Scaffolds in tissue engineering bone and cartilage. Biomaterials 21, 2529–2543.CrossRefGoogle Scholar
  22. 22.
    Hutmacher, D. W., Teoh, S. H., Zein, I., Renawake, M., and Lau, S. (2000) Tissue engineering Research: the engineer’s role. Medical Device Technology 1, 33–39.Google Scholar
  23. 23.
    Jiang, G. and Shi, D. (1997) Coating of hidroxylapatite on highly porous Al2O3 substrate for bone substitutes. J. Biomed. Mater. Res. 43, 77–88.CrossRefGoogle Scholar
  24. 24.
    Maquet, V. and Jerome, R. (1997) Design of macroporous biodegradable polymer scaffolds for cell transplantation, Materials Science Forum 250, 15–42.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Manuela E. Gomes
    • 1
  • Patrícia B. Malafaya
    • 1
  • Rui L. Reis
    • 1
  1. 1.Biomaterials, Biodegradables, and Biomimetics, Department of Polymer EngineeringUniversity of MinhoBragaPortugal

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