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Application of Microscopic Methods for the Detection of Cell Attachment to Polymers

  • John Hunt
  • Deborah Heggarty
Protocol
  • 948 Downloads
Part of the Methods in Molecular Biology™ book series (MIMB, volume 238)

Abstract

Visualizing and quantifying cellular contact with materials is a critical step in the evaluation of cell-material interaction both in vitro and in vivo. To achieve this, a number of techniques are available. The focus of this chapter is the presentation of staining techniques for use with light microscopes, including transmitted, reflected, fluorescence, or laser-scanning confocal. Other visualization techniques and the potential of techniques such as transmission electron microscopes (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM) should not be ignored. But the subject becomes too broad and complex to enable a single protocol to be all-encompassing. However, many of the protocols described can be modified easily for use with TEM and SEM visualization.

Keywords

Focal Contact Fuchsin Acid Cellular Contact Transmitted Visible Light Endogenous Alkaline Phosphatase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Theory and Practice of Histological Techniques (1977) Bancroft, J. and Stevens, A., eds.), Churchill Livingstone, London, pp. 85–94.Google Scholar
  2. 2.
    Theory and Practice of Histological Techniques (1977) Bancroft, J. and Stevens, A., eds.), Churchill Livingstone, London, pp. 95–112.Google Scholar

Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • John Hunt
    • 1
  • Deborah Heggarty
    • 1
  1. 1.UKCTE, Clinical EngineeringUniversity of LiverpoolLiverpoolUK

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