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Microscopic Methods for the Analysis of Engineered Tissues

  • Sally Roberts
  • Janis Menage
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 238)

Abstract

Examination of engineered tissues is clearly necessary to evaluate their properties and determine how close they are to those of the original tissue being engineered. Microscopy encompasses a group of techniques that allows the assessment of many parameters, including information on the cells themselves, on their viability, density, proliferation status, morphology, their capacity for protein synthesis, and their cell activity. Microscopy can also be utilized to follow the structure of any scaffold used and to determine whether it is broken down. In addition to examining how the cells interact with it, the scaffold can be examined to establish whether they adhere successfully, which molecules are involved, and whether the cells go on to produce any matrix—and if so, whether it replaces the original scaffold or integrates with it and builds on it.

Keywords

Freeze Section Neutral Buffer Formalin Fluorescein Diacetate DEPC Water Total Message 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Sally Roberts
    • 1
  • Janis Menage
    • 1
  1. 1.Centre for Spinal StudiesRobert Jones and Agnes Hunt Orthopaedic HospitalOswestryUK

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