Biopolymer Methods in Tissue Engineering pp 159-170 | Cite as
Bioreactor Culture Techniques for Cartilage-Tissue Engineering
Protocol
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Abstract
Tissue engineering is a major focus of biotechnological research today, with the expectation that this type of technique will ultimately transform medical practice (1). The most ambitious tissue-engineering schemes assume that specific tissues and organs will be restored, in a multistage fabrication procedure. For example, cells derived from the patient may be processed to increase the total number available, seeded into a suitable three-dimensional (3D) resorbable scaffold and further processed in vitro to induce the elaboration of neo-tissue prior to implantation (2).
Keywords
Calcium Chloride Solution Trypan Blue Solution Rotate Wall Vessel Rotary Cell Culture System Static Culture Condition
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© Humana Press Inc. 2004