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Chondrocyte Isolation, Expansion, and Culture on Polymer Scaffolds

  • Aileen Crawford
  • Sally C. Dickinson
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 238)

Abstract

The chondrocyte is responsible for maintaining the integrity of the complex extracellular matrix (ECM) of articular cartilage. Therefore, chondrocytes directly control the biomechanical properties of the tissue that enable articular cartilage to bear cyclical compressive loading (1). The ultimate goal of articular cartilage engineering is to produce a cartilage construct with identical structure and properties to native tissue. Although this goal has not yet been achieved, many advances have been made in understanding the biology of the extracellular cartilage matrix (1|–5).

Keywords

Articular Cartilage Polymer Scaffold Spinner Flask Chondrocyte Culture Expansion Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Aileen Crawford
    • 1
  • Sally C. Dickinson
    • 2
  1. 1.School of Clinical DentistryUniversity of SheffieldSheffieldUK
  2. 2.Department of Academic RheumatologyUniversity of Bristol, Southmead HospitalBristolUK

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