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Cell Seeding of Polymer Scaffolds

  • Gordana Vunjak-Novakovic
  • Milica Radisic
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 238)

Abstract

Engineered tissues could form the basis for novel therapies for millions of patients who suffer from the loss of tissue or its function (1), and be used for in vitro studies of tissue development and normal and pathological function. One approach to tissue engineering involves seeding of a high density of uniformly distributed cells on three-dimensional (3D) polymeric scaffolds, and cultivating the resulting cell-polymer constructs under conditions that permit the formation of functional tissues (2). The scaffold provides a defined structure for cell attachment and tissue development, and the bioreactor provides control over the biochemical and physical factors in the cell environment.

Keywords

Cardiac Myocytes Cell Seeding Porous Scaffold Embryonic Chick Polymer Scaffold 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Gordana Vunjak-Novakovic
    • 1
  • Milica Radisic
    • 2
  1. 1.Harvard-MIT Division of Health Sciences and TechnologyMITCambridgeUSA
  2. 2.Department of Chemical EngineeringMITCambridge

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