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Cell Seeding of Polymer Scaffolds

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Biopolymer Methods in Tissue Engineering

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 238))

Abstract

Engineered tissues could form the basis for novel therapies for millions of patients who suffer from the loss of tissue or its function (1), and be used for in vitro studies of tissue development and normal and pathological function. One approach to tissue engineering involves seeding of a high density of uniformly distributed cells on three-dimensional (3D) polymeric scaffolds, and cultivating the resulting cell-polymer constructs under conditions that permit the formation of functional tissues (2). The scaffold provides a defined structure for cell attachment and tissue development, and the bioreactor provides control over the biochemical and physical factors in the cell environment.

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Author information

Authors and Affiliations

  1. Harvard-MIT Division of Health Sciences and Technology, MIT, Cambridge, MA, USA

    Gordana Vunjak-Novakovic

  2. Department of Chemical Engineering, MIT, Cambridge, MA

    Milica Radisic

Authors
  1. Gordana Vunjak-Novakovic
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  2. Milica Radisic
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Editor information

Editors and Affiliations

  1. Centre for Biomaterials and Tissue Engineering, School of Clinical Dentistry, University of Sheffield, Sheffield, UK

    Anthony P. Hollander

  2. Avon Orthopaedic Centre, Southmead Hospital, University of Bristol Academic Rheumatology, Bristol, UK

    Paul V. Hatton

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© 2004 Humana Press Inc.

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Vunjak-Novakovic, G., Radisic, M. (2004). Cell Seeding of Polymer Scaffolds. In: Hollander, A.P., Hatton, P.V. (eds) Biopolymer Methods in Tissue Engineering. Methods in Molecular Biology™, vol 238. Humana Press. https://doi.org/10.1385/1-59259-428-X:131

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  • DOI: https://doi.org/10.1385/1-59259-428-X:131

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-967-4

  • Online ISBN: 978-1-59259-428-3

  • eBook Packages: Springer Protocols

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