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References
Klebanoff, S. J. (1970) Myeloperoxidase: contribution to the microbicidal activity of intact leukocytes. Science 169, 1095–1097
Ames, B. N., Gold, L. S., and Willett, W. C. (1995) The causes and prevention of cancer. Proc. Natl. Acad. Sci. USA 92, 5258–5265
Heinecke, J. W. (1997) Pathways for oxidation of low density lipoprotein by myeloperoxidase: tyrosyl radical, reactive aldehydes, hypochlorous acid and molecular chlorine. Biofactors 6(2), 145–155.
Weiss, S. J. (1989) Tissue destruction by neutrophils. N. Engl. J. Med. 320, 365–376.
Heinecke, J. W., Hsu, F. F., Crowley, J. R., Hazen, S. L., Leeuwenburgh, C., Mueller, D. M., et al. (1999) Detecting oxidative modification of biomolecules with isotope dilution mass spectrometry: sensitive and quantitative assays for oxidized amino acids in proteins and tissues. Methods Enzymol. 300, 124–134.
Frost, M. T., Halliwell, B., and Moore, K. P. (2000) Analysis of free and protein-bound nitrotyrosine in human plasma by a gas chromatography/mass spectrometry method that avoids nitration artifacts. Biochem. J. 345, 453–458.
Gaut, J. P., Byun, J., Tran, H. D., and Heinecke, J. W. (2002) Artifact-free quantification of free 3-chlorotyrosine, 3-bromotyrosine, and 3-nitrotyrosine in human plasma by electron capture-negative chemical ionization gas chromatography mass spectrometry and liquid chromatography-electrospray ionization tandem mass spectrometry. Anal. Biochem. 300, 252–259.
Oury, T., Tatro, L., Ghio, A., and Piantadosi, C. (1995) Nitration of tyrosine by hydrogen peroxide and nitrite. Free Radic. Res. 23(6), 537–547.
Gaut, J. P., Byun, J., Tran, H. D., et al. (2002) Myeloperoxidase produces nitrating oxidants in vivo. J. Clin. Invest. 109, 1311–1319.
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Gaut, J.P., Byun, J., Heinecke, J.W. (2003). Gas Chromatography-Mass Spectrometric Analysis of Free 3-Chlorotyrosine, 3-Bromotyrosine, Ortho-Tyrosine, and 3-Nitrotyrosine in Biological Fluids. In: Hensley, K., Floyd, R.A. (eds) Methods in Biological Oxidative Stress. Methods in Pharmacology and Toxicology. Humana Press. https://doi.org/10.1385/1-59259-424-7:87
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DOI: https://doi.org/10.1385/1-59259-424-7:87
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