Electron Paramagnetic Resonance Spin-Labeling Analysis of Synaptosomal Membrane Protein Oxidation

  • D. Allan Butterfield
Part of the Methods in Pharmacology and Toxicology book series (MIPT)


Synaptosomal protein oxidation, most often indexed by increased protein carbonyl content (1,2), is one biomarker of oxidative stress in brain (2). Protein carbonyls are detected by UV-Vis spectrometric, fluorescence, or immunochemical methods (2), and are increased in a variety of oxidative stress disorders of the brain. Among the latter are Alzheimer’s disease (AD) (3) and animal models of AD (4), Huntington’s disease (HD) (5,6), and accelerated aging (7).


Electron Paramagnetic Resonance Electron Paramagnetic Resonance Spectrum Glutamine Synthetase Spin Label Electron Paramagnetic Resonance Spectrometer 
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Copyright information

© Humana Press Inc.,Totowa, NJ 2003

Authors and Affiliations

  • D. Allan Butterfield
    • 1
  1. 1.Sanders-Brown Center on Aging Center of Membrane Sciences Department of ChemistryUniversity of KentuckyLexington

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